Hopkins Marine Station Student Paper

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(full paper is archived in the Miller Library)

Title: Cellulase treatment of Botryllus eggs removes the barrier to self-fertilization
Student Author(s): Levenson, Amy
Faculty Advisor(s): Epel, David
Pages: 15
Location: Final Papers Biology 175H
Date: June 1981
Keywords: ascidians
Abstract: The present work presents a successful new technique allowing selective dechorionation and removal of follicle cells of the Botryllus egg. With the aid of this technique the phenomenon of colonial self-sterility was examined. The localization of the barrier to self fertilizaiton in the chorion or follicle cells is documented with an increased degree of certainty and detail.