Hopkins Marine Station Student Paper

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(full paper is archived in the Miller Library)

Title: Studies on the induction of the phosynthate release factor in Elysia hedgpethi
Student Author(s): Milkowski, Deborah
Faculty Advisor(s): Epel, David
Pages: 15
Location: Final Papers Biology 175H
Date: June 1988
Keywords: biological studies on gastropods
Abstract: After 15 minutes, 11% of the photosynthate fixed by isolated chloroplasts of Codium fragile was released to the control medium. The percentage released remained constant over a five hour period. Chloroplasts incubated in homogenates of C. fragile did not release a greater percentage of fixed carbon over chloroplasts incubated in the control medium. Extracts from Elysia hedgpethi that were starved for 16 days also did not induce the release of photosynthate from isolated chloroplasts. Further experiments were done using E. hedgpethi that were starved for 22 days and then re-fed C. fragile until their green color was restored (7 days). Homogenates of the re-fed slugs again showed no increase in the percentage of fixed carbon released. In one experiment using extracts from Aplysiopsis smithi, 52.3% of the photosynthate was released fromisolated chloroplasts, over 2.5 times the percentage released to the control medium.