Hopkins Marine Station Student Paper

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(full paper is archived in the Miller Library)

Title: FCP gene family of Macrocystis pyrifera (Giant Kelp): development of gene specific probes
Student Author(s): Tostado, Marina L.
Faculty Advisor(s): Clendennen, Stephanie K.
Pages: 11
Location: Final Papers Biology 175H
Date: June 1992
Abstract: Eight genes in Macrocystis pyrifera code for the FCP protein. Each gene has similar coding regions but very different noncoding regions (dissimilar sequences and sizes). From this gene family three genes, 2A, 4B, and 4C were studied. Gene specific primers were made and challenged with positive results. These primers were used in PCR reactions to make gene specific probes. The gene specificities of the probes were tested using Southern Blot Analysis concluding with supporting results. A light regulation experiment using RNA obtained from incubated kelp blades which were analyzed on a Northern Blot using the radioactive gene specific probes was conducted but its results were ambiguous.