(full paper is archived in the Miller Library)
Title: A protein comparison between aposymbiotic and symbiotic Anthopleura elegantissima
Student Author(s): Lam, Alan S.
Faculty Advisor(s): Levine, R.P.
%E Weis, V.M.
Location: Final Papers Biology 175H
Date: June 1994
Abstract: In the Monterey Bay, there are two types of Anthopleura elegantissima: symbiotic and aposymbiotic. Symbiotic anemones have a relationship with unicellular dinoflagellates. Algae are singly bound in membrane vacuoles within the endoderm of the anemone. Aposymbiotic anemones do not have this association and probably do not have the metabolic functions that are part of the symbiosis. Because of the differences in these anemones, the hypothesis was established that there were protein differences between aposymbiotic and symbiotic anemones. Qualitatively, both membrane and soluble proteins in both anemones were analogous. Quantitatively, however, disparate soluble protein quantities of sizes 106 kD and 14 kD were found. The 14 kD protein was more prevelant in the symbiotic anemone based on optical density readings taken from a scanned gel (0.292 vs. 0.057 Trace Optical Density x mm); the 106 kD protein had a greater expression in the aposymbiotic anemone (0.25 vs. 0.17). Further testing is needed to identify these proteins.