Hopkins Marine Station Student Paper

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Title: Production of p57 by Renibacterium salmoninarum within rainbow trout macrophages
Student Author(s): Dhawan, Archana
Faculty Advisor(s): Levine, Paul
Pages: 19
Location: Final Papers Biology 175H
Date: June 1995
Abstract: Renibacterium salmoninarum is an intracellular pathogen that survives within the macrophages of salmonids. The ability of R. salmoninarum to produces p57, a major surface antigen of the bacterium, while inside rainbow trout macrophages was determined. R. salmoninarum was incubated with rainbow trout macrophages isolated from the head kidney and spleen, and phagocytosis occurred as demonstrated by immunofluorescence. Macrophages were then lysed and a Western blot was run on the lysate, to determine the presence of p57. Macrophages incubated with only L-15 media showed no p57 production nor did macrophages incubated with killed R. salmoninarum. Macrophages incubated with R. salmoninarum showed the presence of p57, thus indicating that p57 was produced by R. salmoninarum while inside the trout macrophages. These results suggest that p57 may play a role in the intracellular survival of R. salmoninarum.