(full paper is archived in the Miller Library)
Title: Enzyme thermal stabilities among three vertically-distributed species of the genus Tegula
Student Author(s): Waites, Hilary
Faculty Advisor(s): Somero, George
Location: Final Papers Biology 175H
Date: June 1997
Abstract: Protein thermal stability in archeogastropod snails distributed along a vertical intertidal/subtidal gradient was investigated to determine whehter a correlation exists between habitat temperature and enzyme stability. Assays were run for the following enzymes: malate dehydrogenase (MDH), pyruvate kinase (Pk), aldolase, glutamate dehydrogenase (GDH), and aspartate aminotransferase (AAT). A positive correlation between habitat temperature and enzyme stability was found in MDH and PK, a negative correlation was found in GDH and AAT, and no correlation was observed in aldolase. Since thermal stability is a macroscopic measurement of protein structure, further studies involving protein function (kinetics) and the effect of the intracellular microenvironment are needed to further elucidate the relationship between habitat temperature and protein adaptation.