(full paper is archived in the Miller Library)
Title: Proliferation and migration of brain cells in the zebrafish, Danio rerio
Student Author(s): Curley, Nina
Faculty Advisor(s): Thompson, Stuart
Location: Final papers Biology 175H
Date: June 2003
Abstract: The zebrafish Danio rerio is very useful for the study of cell proliferation in the brain, not only because it has been entirely genetically sequenced, but also because teleosts have remarkable abilities to regenerate parts of their central nervous system after injury. In adult zebrafish, neurogenesis has been documented predominantly in the cerebellum, and in ventricular zones, especially in the telencephalic ventricle in the forebrain. Neurogenesis in the ventricular zones implies that the cells are turning over and remaining progenitor cells; however this study examines whether these cells are differentiating. Discovering a path of differentiaton would lend insight into the regulation of regeneration. This study focuses on possible migration of progenitor cells from the periventricular zone to the optic tectum in zebrafish embryos as they develop, using BrdU to label dividing cells and confocal microscopy to image these cells. The results reveal a smattering of nuclei throughout the optic tectum and encephalon, suggesting migration outward from ventricular areas.