(full paper is archived in the Miller Library)
Title: Glutamate calcium signals in proliferating cells of the adult zebrafish central nervous system
Student Author(s): Merksamer, Philip
Faculty Advisor(s): Thompson, Stuart
Location: Final Papers Biology 175H
Date: June 2003
Abstract: The periventricular zone (PVZ) of the adult zebrafish central nervous system contains undifferentiated, proliferating cells consistent with the properties of neural progenitors. Past studies suggest that neuronal projections may link the cells of the optic tectum to those in the PVZ. This study explores the pharmacological profile of the cells in the PVZ and examines the hypothesis that cells in the optic tectum may communicate with those in the PVZ through conventional neurotransmitters. Living optic tectum slices were imaged using time-lapse confocal microscopy during application of the excitatory neurotransmitters, glutamate and acetylcholine, and glutamate antagonists. The application of glutamate induced intracellular calcium changes in the cells of the PVZ. The glutamate antagonist, APV, blocked these signals.