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American Chemical Society, Division of Chemical Information, Education Committee: Computer Searching of Chemical Abstracts: Biochemistry Sample Search.

Biochemistry Sample Search

Search Topic:

Find references on the use of antibodies to study acetylcholine receptors.

Strategy:

  1. Find Registry Number for Acetylcholine
  2. Key terms:
    • Acetylcholine or its Registry Number
    • receptor# (The # symbol acts as one or zero characters; this retrieves receptor, receptors)
    • antibod? (retrieves antibody, anitbodies)
  3. Link terms with (s) operator for greater precision
  4. Display scan a few references to check for additional relevant terms.
  5. Add additional synonyms for acetycholine-related receptors: cholinergic, nicotinic, muscarinic

Teaching Points:

Registry File:

CA File:

Search Strategy Table

Search Steps Set Number Number of Documents
File reg
e acetylcholine/cn
s E3 L1 1
d L1 ide 1
file ca
s L1 or acetylcholine L2 41310
s receptor# L3 319187
s antibod? L4 206193
s L2 (s) L3 (s) L4 L5 757
d L5 scan
s muscarinic or nicotinic or cholinergic L6 49216
s (L2 or L6) (s) L3 (s) L4 L7 938
s L7 not L5 L8 181
d L8 ibib 1
log y

Sample Search

Search in Registry File for Registry Numbers for acetylcholine

=> file reg

FILE 'REGISTRY' ENTERED AT 17:36:18 ON 04 JUN 96 USE IS SUBJECT 
TO THE TERMS OF YOUR CUSTOMER AGREEMENT COPYRIGHT (C) 
1996 American Chemical Society (ACS)

STRUCTURE FILE UPDATES:  31 MAY 96   HIGHEST RN 176894-09-0
DICTIONARY FILE UPDATES:  3 JUN 96   HIGHEST RN 176894-09-0

TSCA INFORMATION NOW CURRENT THROUGH DECEMBER 1995

Expand on acetylcholine in the Chemical Name (cn) field to verify the form of the name of the Registry file

=> e acetylcholine/cn

E1           1     ACETYLCHLOROSULFISOMIDINE/CN
E2           1     ACETYLCHOLIN-RETARD/CN
E3           1 --> ACETYLCHOLINE/CN
E4           1     ACETYLCHOLINE + ESERINE/CN
E5           1     ACETYLCHOLINE .BETA.-RESORCYLATE/CN
E6           1     ACETYLCHOLINE 2-NITROBENZOATE/CN
E7           1     ACETYLCHOLINE 3-CHLOROBENZOATE/CN
E8           1     ACETYLCHOLINE 3-PHENYLPROPIONATE/CN
E9           1     ACETYLCHOLINE 4-NITROBENZOATE/CN
E10          1     ACETYLCHOLINE ACETYLHYDROLASE/CN
E11          1     ACETYLCHOLINE BENZENESULFONATE/CN
E12          1     ACETYLCHOLINE BENZOATE/CN

Search the E# from the expand list to save retyping acetylcholine

=> s e3

L1           1 ACETYLCHOLINE/CN

Display answer 1 of set one in ide format

=> d l1 ide 1

L1   ANSWER 1 OF 1  REGISTRY  COPYRIGHT 1996 ACS
RN   51-84-3  REGISTRY
CN   Ethanaminium, 2-(acetyloxy)-N,N,N-trimethyl- (9CI)  
(CA INDEX NAME)
OTHER CA INDEX NAMES:
CN   Choline acetate (ester) (8CI)
CN   Choline, acetate (7CI)
OTHER NAMES:
CN   Acetyl choline ion
CN     Acetylcholine
CN   Acetylcholine cation
CN   Acetylcholinium
CN   ACh
CN   Choline, acetyl-
FS   3D CONCORD
MF   C7 H16 N O2
CI   COM
LC   STN Files:   AIDSLINE, ANABSTR, BEILSTEIN*, 
BIOBUSINESS, BIOSIS, CA, CABA, CANCERLIT, CAOLD, 
CAPLUS, CAPREVIEWS, CASREACT, CEN, CHEMLIST, CIN, 
CJACS, CSNB, DDFU, DRUGU, EMBASE, GMELIN*, IFICDB,
 IFIPAT, IFIUDB, IPA, MEDLINE, NAPRALERT, PNI, PROMT, 
RTECS*, SPECINFO, TOXLINE, TOXLIT, USAN, USPATFULL, 
VETU  (*File contains numerically searchable property data)
Other Sources:   EINECS**
(**Enter CHEMLIST File for up-to-date regulatory information)


Me3+N        CH2       CH2      OAc
    .........   .......  .......

32 REFERENCES IN FILE CAPREVIEWS
21223 REFERENCES IN FILE CA (1967 TO DATE)
85 REFERENCES TO NON-SPECIFIC DERIVATIVES IN FILE CA
21260 REFERENCES IN FILE CAPLUS (1967 TO DATE)
1 REFERENCES IN FILE CAOLD (PRIOR TO 1967) 

Transfer to the CA file to search for relevant articles

=> file ca

FILE 'CA' ENTERED AT 19:06:11 ON 24 JUN 96 USE IS SUBJECT 
TO THE TERMS OF YOUR CUSTOMER AGREEMENT COPYRIGHT (C) 
1996 AMERICAN CHEMICAL SOCIETY (ACS)

FILE COVERS 1967 - 19 Jun 1996 (960619/ED)  VOL 124 ISS 26

Search both the L# from the Registry file (for the Registry number) and the name acetylcholine

=> s l1 or acetylcholine

         21481 L1
         40657 ACETYLCHOLINE
L2       41310 L1 OR ACETYLCHOLINE

Search the other two terms individually for maximum flexibility in combining them.

=> s receptor#

L3      319187 RECEPTOR#

=> s antibod?

L4      206193 ANTIBOD? 

Combine the terms with the (s) “same sentence” operator for greater specificity than with AND

=> s L2 (s) L3 (s) L4

L5         757 L2 (S) L3 (S) L4

Display answer 3 of L5 in ibib format to give hints for other additional search terms.

=> d L5 ibib 3

L5   ANSWER 3 OF 757  CA  COPYRIGHT 1996 ACS

ACCESSION NUMBER: 124:286753  CA
TITLE:            A synthetic peptide corresponding to the second 
                  extracellular loop of the human M2 acetylcholine  
                  receptor induces pharmacological and morphological   
                  changes in cardiomyocytesby active immunization  
                  after 6 months in rabbits
AUTHOR(S):        Fu, Michael L. X.; Schulze, Wolfgang; Wallukat, 
                  Gerd; Hjalmarson, Aake; Hoebeke, Johan
CORPORATE SOURCE: Wallenberg Laboratory, Sahlgren's  
                  Hospital, Goeteborg, S-413 45, Swed.
SOURCE:           Clin. Immunol. Immunopathol. (1996), 78(2),   
                  203-7 CODEN: CLIIAT;
ISSN:             0090-1229
DOCUMENT TYPE:    Journal
LANGUAGE:         English

Search the additional terms found for acetylcholine-related receptors

=> s muscarinic or nicotinic or cholinergic

         14983 MUSCARINIC
         14753 NICOTINIC
         29900 CHOLINERGIC
L6       49216 MUSCARINIC OR NICOTINIC OR CHOLINERGIC

Combine this set of answers with the set for “acetycholine” using OR, then combine with “receptor#” and “antibod?”

=> s (L2 or L6) (s) L3 (s) L4

L7         938 (L2 OR L6) (S) L3 (S) L4

Use Not to identify the new records not found in the search. Note that 181 more references were found with the added search terms.

=> s L7 not L5

L8         181 L7 NOT L5

Displays answer 1 of L8 in indented bibliographic format

=> d L8 ibib 1

L8   ANSWER 1 OF 181  CA  COPYRIGHT 1996 ACS

ACCESSION NUMBER:        124:340916  CA
TITLE:                   Target specific screens and their use for          
                         discovering small organic molecular 
                         pharmacophores
INVENTOR(S):             Blume, Arthur J.
PATENT ASSIGNEE(S):      Dgi Technologies, Inc., USA
SOURCE:                  PCT Int. Appl., 202 pp.
CODEN: PIXXD2

NUMBER                  DATE
--------------          ------
PATENT INFORMATION:      WO 9604557 A2           960215
DESIGNATED STATES:       W:  AM, AT, AU, BB, BG, BR, 
BY, CA, CH, CN, CZ, DE, DK, EE, ES, FI, GB, GE, HU, IS, 
JP, KE, KG, KP, KR, KZ, LK, LR, LT, LU, LV, MD, MG, MN, 
MW, MX, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, TJ, 
TM, TT RW: AT, BE, BF, BJ, CF, CG, CH, CI, CM, DE, DK, 
ES, FR, GA, GB, GR, IE, IT, LU, MC, ML, MR, NE, NL, PT, 
SE, SN, TD, TG 
APPLICATION INFORMATION: WO 95-US10182           950802
PRIORITY APPLN. INFO.:   US 94-286084            940803
                         US 95-473105            950607
DOCUMENT TYPE:           Patent
LANGUAGE:                English

Log off in one step

=> log y
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