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Stanford Report, April 16, 2003

Researchers solve mystery behind skull plate fusion

By KRISTA CONGER

Medical center researchers have identified a protein responsible for ensuring correct skull growth in newborn mice. The protein, called Noggin, inhibits fusion of bony plates in the skull until developmentally appropriate. The scientists hope that Noggin may one day replace surgery as a way to treat premature skull fusion in infants.

"About one in 2,000 children has growth plates in their skull that fuse prematurely," said Michael Longaker, MD. "The brain is rapidly expanding in size during the first two years of life. If the brain’s container — the skull — can’t expand in a similar fashion, you have a big problem." Left untreated, the condition can lead to mental retardation, blindness and seizures, as well as a severely misshapen head.

Longaker, a pediatric craniofacial surgeon at Lucile Packard Children’s Hospital and a professor of surgery at the medical school, can correct the defect by removing sections of fused bone from an infant’s skull. But the operation is complex. And because it’s difficult to accurately predict how much room is needed for expansion, the procedure may need to be repeated as the brain grows.

Longaker and his colleagues first suspected that the root of the problem was the inappropriate expression of proteins that stimulate bone growth in the skull. They began comparing when and where these proteins were produced, but they started on the wrong track.

"It’s not as simple as promoting bone induction," said Longaker, the principal investigator of the research, which was published in the April 10 issue of Nature. "It turns out that the inhibitors are equally important. We had been missing the point."

The researchers discovered that the bone-promoting proteins are present between all the skull growth plates in mice: those that are actively fusing and those that are not. In contrast, Noggin, a known inhibitor of bone formation, was found only between plates that remained open. The scientists began to suspect that, like a testy chaperone at a high school dance, Noggin keeps the two edges of the skull apart. And like anxious teenagers, the bones reach out to each other when Noggin is removed from the mix.

Noggin’s new role in skull fusion was confirmed dramatically when the scientists injected a Noggin-producing virus between the bone plates along mice foreheads. The plates, which normally would have fused, remained open. This resulted in the animals developing blunt muzzles and wide-set eyes.

Although the results were clear, another mystery needed to be solved. In a seemingly futile loop, the bone-promoting protein required for fusion also induced Noggin production. Then the researchers found that over-expression of another protein, called FGF2, inhibited Noggin expression in cell culture. The finding correlated with the fact that some human disorders characterized by premature skull fusion are caused by mutations in a receptor for FGF2 that kick the protein into overdrive. These mutants also turned off Noggin expression.

"We had been wondering how FGF2, which is involved in promoting new blood vessels, stimulated bone formation," said Longaker. "Now we know it works in part by decreasing the amounts of inhibitors of bone formation."

The scientists are now working to understand how FGF2 decreases Noggin production, with an eye to a less-invasive treatment for infants with premature fusion. But such treatment would likely require early diagnosis of any problems.

"Can Noggin unlock an existing bone bridge? That would be a tall order," said Longaker. "But if premature fusion could be diagnosed before birth, a window of therapy could present itself." He envisions a day when sophisticated prenatal ultrasound or genetic testing could identify infants at risk before fusion began. By injecting Noggin-producing cells or viruses into the breach between growth plates, physicians could stave off inappropriate fusion until the brain finished expanding.

"Unlike some gene therapy challenges, we wouldn’t need to express Noggin for the life of the child," said Longaker. "The first two years would be adequate." Such a treatment would represent a huge leap over existing therapy. "Craniofacial surgery is more of a chisel-and-saw approach," he added. "We’ve had technical improvements, but it hasn’t really evolved. This finding represents a biomolecular approach that may re-establish normal growth and appearance of the skull."

Stephen Warren, MD, of Stanford, also contributed to the work.




From the court to the OR, Longaker plays serious ball (3/13/02)