Flow Cytometry - Cytof
Muscle stem cells (MuSCs) are responsible for the maintenance and regeneration of skeletal muscle mass. The Blau laboratory has characterized adult murine MuSCs by flow cytometry, in conjunction with quantitative in vivo functional assays of their regenerative capacity, by using bioluminescence imaging and assays of twitch and tetanic force.
Although the MuSC population routinely isolated by flow cytometry is highly enriched for myogenic cells, this population is known to be heterogeneous and contain multiple functional subsets. Using single cell mass spectrometry we are now characterizing the heterogeneity of the MuSC population and resolving distinct functional subsets with the goal of identifying the optimal subset for regeneration as well as signaling pathways that go awry in cancer, aging and muscular dystrophy with a view toward developing therapeutic strategies.
* Sacco, A., R. Doyonnas, et al. (2008).Add PDF link
* Bendall, S. C., E. F. Simonds, et al. (2011). Add PDF link
* Bendall and Nolan, Nature Biotechnology, 2012