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Tethered Vesicles Diffusing in Two-Dimensions

Lipid vesicles displaying DNA-lipid A can be tethered via DNA hybridization from a glass supported lipid bilayer displaying the complementary strand, A′.  The tethered vesicles diffuse randomly in the plane parallel to the supported lipid bilayer, as shown in this video taken on an inverted fluorescence microscope.  The colored lines trace the movement of six different vesicles through the video. The diffusion coefficient of tethered vesicles was measured by single particle tracking to be approximately 0.2 μm2/sec.  Vesicles occasionally collide, but are not observed to dock or fuse. 

"Arrays of Mobile Tethered Vesicles on Supported Lipid Bilayers", Chiaki Yoshina-Ishii and Steven G. Boxer, J. Am. Chem. Soc., 125, 3696-3697 (2003). [pdf]

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