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Renewables > Bioenergy

Lignin Management: Optimizing Yield and Composition in Lignin-Modified Plants

Start Date: September 2013


Clint Chapple, Department of Biochemistry, Purdue University; Wout Boerjan, VIB Department of Plant Systems Biology, UGent Department of Plant Biotechnology and Bioinformatics; Claire Halpin and Gordon Simpson, University of Dundee; John Ralph, University of Wisconsin; and Xu ‘Sirius’ Li, North Carolina State University

Annual Reports


  • Lan W., Morreel K., Lu F., Rencoret J., del Rio J.C., Voorend W., Vermerris W., Boerjan W., and Ralph J. Maize tricin-oligolignol metabolites and their implications for monocot lignification. Plant Physiol. 171, in press, (2016).
  • Rinaldi R., Jastrzebshi R., Clough M.T., Ralph J., Kennema M., Bruijnincx P.C.A., Weckhuysen B.M. Paving the way for lignin valorisation: Recent advances in bioengineering, biorefining and catalysis. Angewandte Chemie (English Edition): in press, (2016).
  • Yue F., Lu F., Ralph S.A., Ralph J. Identification of 4–O–5-units in softwood lignins via definitive lignin models and NMR. Biomacromolecules: in press, (2016).
  • Mottiar Y., Vanholme R., Boerjan W., Ralph J., Mansfield S.D. Designer lignins: harnessing the plasticity of lignification. Current Opin. Biotechnol. 37, 190-200, (2016).
  • Anderson, N. A., Tobimatsu, Y., Ciesielski, P. N., Ximenes, E., Ralph, J., Donohoe, B. S., Ladisch, M., and Chapple C. Manipulation of guaiacyl and syringyl monomer synthesis in cad-c cad-d Arabidopsis results in atypical lignin biosynthesis and modified cell wall structure. Plant Cell 27, 2195-2209, (2015).
  • Dima, O., K. Morreel, B. Vanholme, H. Kim, J. Ralph, W. Boerjan, Small glycosylated ligninoligomers are stored in Arabidopsis leaf vacuoles, Plant Cell, 27, 695-710, 2015.
  • Kim, J. I., Dolan, W. L. Anderson N. A., and Chapple, C. Indole glucosinolate biosynthesis limits phenylpropanoid accumulation in Arabidopsis thaliana. Plant Cell, 27, 1529-1546, (2015).
  • Grabber, J.H., Santoro, N., Foster, C.E., Elumalai, S., Ralph, J., and Pan, X. Incorporation of flavonoid derivatives or pentagalloyl glucose into lignin enhances cell wall saccharification following mild alkaline or acidic pretreatments. BioEnergy Research 8, 1391-1400 (2015).
  • Kim, J.I., Ciesielski, P.N., Donohoe, B.S., Chapple, C., and Li, X. Chemically induced conditional rescue of the reduced epidermal fluorescence8 mutant of Arabidopsis reveals rapid restoration of growth and selective turnover of secondary metabolite pools. Plant Physiol., 164, 584-595, (2014).
  • Bao, Z., Benson, S., Cui, Y., Dionne, J.A., Maher, K., Boerjan, W., Halpin, C., Nelson, R., Nichols, D., Ralph, J., and Ramakrishnan, T. S. In search of clean, affordable energy, Oilfield Review, 26, 4-15, (2014).
  • Bonawitz, N.D., Kim, J.I., Tobimatsu, Y., Ciesielski, P.N., Anderson, N.A., Ximenes, E., Maeda, J., Ralph, J., Donohoe, B.S., Ladisch, M., and Chapple C., Disruption of Mediator rescues the stunted growth of a lignin-deficient Arabidopsis mutant, Nature, 509, 376-380, (2014).
  • Tobimatsu, Y., Van de Wouwer, D., Allen, E., Kumpf, R., Vanholme, B., Boerjan, W., Ralph, J. A click chemistry strategy for visualization of plant cell wall lignification, Chem. Commun., 50, 12262- 12265, (2014).
  • Morreel, K., Y. Saeys, O. Dima, F. Lu, Y. Van de Peer, R. Vanholme, J. Ralph, B. Vanholme, W. Boerjan, Systematic structural characterization of metabolites in Arabidopsis via candidate substrate- product pair networks, Plant Cell, 26, 929-945, (2014).
  • Sundin, L., Vanholme, R., Geerinck, J., Goeminne, G., Höfer, R., Kim, H., Ralph, J., and Boerjan W. Mutation of the inducible ARABIDOPSIS THALIANA CYTOCHROME P450 REDUCTASE2 alters lignin composition and improves saccharification, Plant Physiol., 166, 1956-1971, (2014).
  • In search of clean, affordable energy. R. Nolen-Hoeksema, Z. Bao, S. Benson, J. A. Dionne, K. Maher, W. Boerjan, C. Halpin, R. Nelson, D. Nichols, J. Ralph and T. S. Ramakrishnan. Oilfield Review, 2014, 26, in press.
  • Systematic structural characterization of metabolites in Arabidopsis via candidate substrate-product pair networks. K. Morreel, Y. Saeys, O. Dima, F. Lu, Y. Van de Peer, R. Vanholme, J. Ralph, B. Vanholme and W. Boerjan. Plant Cell, 2014, in press.
  • “Visualization of plant cell wall lignification using fluorescence-tagged monolignols. Y. Tobimatsu, A. Wagner, L. Donaldson, P. Mitra, C. Niculaes, O. Dima, J.-I. Kim, N. A. Anderson, D. Loque, W. Boerjan, C. Chapple and J. Ralph. The Plant Journal, 2013, 76, 357-366.”
  • Vanholme, R., Cesarino, I., Rataj, K., Xiao, Y., Sundin, L., Goeminne, G., Kim, H., Cross, J., Morreel, K., Araujo, P., Welsh, L., Haustraete, J., McClellan, C., Vanholme, B., Ralph, J., Simpson, G. G., Halpin, C., and Boerjan W. Caffeoyl shikimate esterase (CSE) is an enzyme in the lignin biosynthetic pathway, Science, 341, 1103-1106, (2013)

Updated July 2016