Heilshorn Biomaterials Group

Materials Science & Engineering Department
Stanford University

    Main    Members     Research     Publications     News     Media     Lab     Support     Links     Courses     Contact    

Widya Mulyasasmita

email: widyam AT stanford DOT edu

Dept: Bioengineering

            B.S.  Materials Science & Engineering;                                      Bioengineering, UC Berkeley

Accurate in vitro recapitulation of complex biological phenomena such as tissue morphogenesis, tumorigenesis, and stem cell differentiation requires a truly 3D environment. Common physical hydrogel-based 3D cell encapsulation platforms such as matrigel and collagen require a shift in environmental conditions (e.g. temperature, pH) to initiate the sol-gel transition, which may be detrimental to cells.  By exploiting binding interactions between tandem repeats of the WW domain and its proline-rich ligand, we have designed a two-component physical hydrogel system that undergoes a sol-gel transition simply upon mixing. Furthermore, the addition of soluble proline-rich peptides results in competitive binding that causes rapid gel dissolution, thereby facilitating easy cell recovery under mild, non-enzymatic conditions.  Molecular engineering of the recognition domains is also being pursued to achieve independent control over mechanical and biochemical cues imparted to the cell.                                                                                                                                                                   

Materials Science & Engineering DepartmentStanford University

Updated 8/09