Vladimir Kesler

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BEng, McGill University, 2016

Email: vkesler@stanford.edu

Wireless, implantable system for aptamer-based biosensing

Structure switching aptamer based sensors have been shown to be a promising tool for real time tracking of biomarkers [1]. By attaching a redox-active marker to a sequence of nucleotides, one can obtain a real-time molecular sensor for a wide variety of targets. These sensors are operated using square-wave voltammetry, a method that requires a potentiostat. However, conventional potentiostats are both bulky and more power-hungry than is feasible for an implantable system. This prevents studies from using freely moving animals, and rules out eventual human applications such as real-time health monitoring. In collaboration with the H. Tom Soh Laboratory, this project seeks to develop an implantable system to operate the sensors, collect data, and communicate with an external device.

Square wave voltammetry for aptamer-based sensors [1][2]

A board level platform, VIPER, is being developed for initial studies. This system will leverage the form-factor and protocols of modern pacemakers. Housed in a pacemaker enclosure, the system will be based around a wireless MCU with a separate analog front-end sensing module. The pacemaker leads will be modified to become a substrate for the aptamer-based sensors. The modular design will allow the system to be modified for other applications in the future.

Modular VIPER block diagram

[1] B. Ferguson, D. Hoggarth, D. Maliniak, K. Ploense, R. White, N. Woodward, K. Hsieh, A. Bonham, M. Eisenstein, T. Kippin, K. Plaxco and H. Soh, "Real-Time, Aptamer-Based Tracking of Circulating Therapeutic Agents in Living Animals", Science Translational Medicine, vol. 5, no. 213, pp. 213ra165-213ra165, 2013.

[2] Louis Ramaley and Matthew S. Krause,"Theory of square wave voltammetry", Analytical Chemistry 1969 41 (11), 1362-1365, DOI: 10.1021/ac60280a005

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