Mass Cytometry

Mass Cytometry

Mass cytometry is a mass spectrometry technique based on inductively coupled plasma mass spectrometry combined with mass tags that allows accurate measuring of various molecular species on single cells in a highly multiplexed fashion.


Multiplexed Ion Beam Imaging

Multiplexed Ion Beam Imaging (MIBI)

We have developed a method that uses secondary ion mass spectrometry to image antibodies that are tagged with isotopically pure elemental metal reporters.




Phospho Flow

Phospho flow

Phospho-specific flow cytometry, or phospho flow, measures the phosphorylation state of intracellular proteins at the single cell level.





What we do

We use multiparametric single-cell analysis to study hematopoiesis, cancer and leukemia, autoimmunity and inflammation. We also develop computational approaches for network and systems immunology.  Our most recent efforts are focused on a single cell analysis advance using a mass spectrometry-flow cytometry hybrid device, the so-called “CyTOF”. 

The approach uses an advanced ion plasma source to determine the levels of tagged reagents bound to cells—enabling a vast increase in the number of parameters that can be measured per cell.  Our laboratory has already begun a large scale mapping of the hematopoietic hierarchy in healthy human bone marrow at an unprecedented level of detail.

We are working to enable a deeper understanding not only of normal immune function, but also detailed substructures of leukemias and solid cancers as well as autoimmunity and pathogen effects upon the immune system.


Professor Garry Nolan

 

Professor, Microbiology & Immunology - Baxter Laboratory

Member, Bio-X Member, Child Health Research Institute Member, Stanford Cancer Institute

Full bio

Quick Contact

Primary Contacts:
For procedural questions regarding MTAs, Phoenix cells, or directions to the lab, please contact Howard Guss.
Executive Assistant:
Howard Guss
Tel: (650) 725-7002
Fax: (650) 723-2383
howardg(at)stanford(dot)edu




Internal Website
Legacy Website