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Dept of Molecular Pharmacology
Dept of Microbiology & Immunology

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Companies and industrial institutes should direct inquiries by e-mail or letter directly to:

Khoua Vang kvang@leland.stanford.edu

Instructions and important information needed in order to obtain cell lines or plasmid vectors from the Nolan Lab - please print this page

Owing to the volume of requests for these cells we have now provided the ATCC with the Phoenix ECO and AMPHO cells for further distribution. Authorization from the Nolan laboratory in the form of a written signature from Dr. Nolan is necessary to obtain the cells from the ATCC. The cells have been declared free of mycloplasma and other tested pathogens by the ATCC

If you want the cells with as little delay as possible follow these steps carefully. Companies and industrial institutes should direct inquiries by e-mail or letter directly to Khoua Vang kvang@leland.stanford.edu

  1. Begin by downloading the desired MTA form.

  2. Read the agreements CAREFULLY and note the restrictions.

  3. The Principal Investigator's name is required to be the name on the agreement to whom the cells are addressed, not the post-doc or graduate student.

  4. The LABORATORY HEAD should sign the agreement and then get a co-signature from an appropriate and authorized Institute Official. Forms signed by postdoctoral fellows or graduate students will be set aside and no further action will be taken.

  5. Write a one or two paragraph explanation of your intended use of the cells.

  6. Fully complete the front page of the the MTA including name, address (no P.O. Boxes), fax and phone and e-mail address.

  7. Return the signed Material transfer Agreement along with the written paragraph to the Nolan lab.

  8. At the Agreed and Accepted lines both signature and title are required.

  9. Authorization will then be provided to the ATCC by Dr. Nolan. Authorizations will be made on a weekly basis. You may then contact the ATCC who will ship you the cells.

  10. When ordering the cells from the ATCC the name of the recipient must be the same name on the MTA (i.e. the laboratory head).

We WILL NOT FINALIZE MTAS without an AUTHORIZED INSTITUTE OFFICIAL'S signature and a WRITTEN PARAGRAPH on intended use.

This latter paragraph provides liability assurance. No MTAs have ever been denied to date on the basis of scientific content.

Incomplete forms will have no further action taken on them.

Downloadable pdf versions of MTA's (click on the link to download the pdf):

  • Phoenix Ampho & Eco cell lines - second generation retroviral producer lines (MMULV-based).

  • PhoenixGP cell line, 293T-derived containing only gag-pol for VSV-G and other pseudotyping.

  • VSV-G expression plasmid.

  • Felix FIV-based (Feline Immunodeficiency) vectors for delivery to non-dividing cells.

  • Helix HIV-1 based (Human Immunodeficiency) vectors for delivery to non-dividing cells.

  • pBMN-LacZ vector - a pBabeMN based retroviral construct with the LacZ gene.

  • pBMN-i-EGFP vector - a pBabeMN based retroviral construct with the EMCV IRES upstream from the humanized/enhanced GFP (EGFP).

  • pBMN-z-i-neo vector - a pBabeMN based retroviral construct with the EMCV IRES upstream from the Neomycin resistance gene

  • LZRS-pBMN-LacZ vector - EBV/retroviral vector containing orip and EBNA-1 elements, the gene unit PGK-1/Puromycin resistance and a retrovirus expressing the LacZ gene.

  • pBMN-hTERT vector - a pBabeMN based retroviral construct with human TERT

  • Jurkat.EcoR cell line - Jurkat T lymphocytes which express ecotropic receptor of mouse leukemia virus.

 

 

    

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