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Dept of Molecular Pharmacology
Dept of Microbiology & Immunology

> Nolan Lab

           
 
           

 

   

Lab Members (name links to addl info):

Dr. Garry Nolan, Ph.D.

Greg Behbehani, Ph.D.
Sean Bendall, Ph.D.
Zach Bjornson
Robert Bruggner, B.S.
Tyler Burns
Shih-Yu Chen, Ph.D.
Matthew Clutter, Ph.D.
Kara Davis, D.O.
Wendy J. Fantl, Ph.D.
Harris Fienberg, B.S.
Rachel Finck
Gabi Fragiadakis
Brice Gaudilliére, M.D., Ph.D.
Yury Goltsev, Ph.D.
Howard Guss
Matthew Hale, Ph.D.
Faye Y Hsu, Ph.D.
Astraea Jager
Gina Jager
Nikesh Kotecha, Ph.D.
Peter Krutzik, Ph.D.
Cynthia Lorang
Rosalyn McCambridge
Veronica Muñoz, Ph.D.
Karen Sachs, Ph.D.
Erica Savig

Erin Simonds, Ph.D.
Matt Spitzer
Angelica Trejo
Eli Zunder Ph.D.
Please click here for alumni information.
































Member Information:


Greg Behbehani, Ph.D
Position: Postdoctoral Fellow
Education: B.S., Biology, 1996, University of Cincinnati; M.D., 2005, University of Cincinnati College of Medicine; Ph.D., Molecular Genetics, Biochemistry and Microbiology, 2007, University of Cincinnati College of Medicine
Specialties: Hematology and Oncology
email:
Field: Hematology and Oncology (translational research)
Project(s): I am interested in using single cell flow cytometry methods to analyze chemotherapy response in leukemia cell lines in order to predict therapy response and determine the optimal chemotherapy regimen for individual patients.

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Dr. Sean Bendall, Ph.D
Position: Postdoctoral Fellow
Education: BSc - Biochemistry & Microbiology, Univ. of Victoria, Canada; PhD - Biochemistry, Univ. of Western Ontario, Canada
Specialties: Protein Biochemistry, Mass Spectrometry, Stem Cell Biology, ICP-MS based flow cytometry (CyToF)
email:
Field: Method Development, Phospho Flow Cytometry, Stem Cell and Cancer Biology
Project(s): Interfacing phospho-flow protocols with high content ICP-MS based flow cytometry (CyToF). Using single cell phospho-flow to characterize regulatory signaling network status in rare cancer and stem cell populations and correlate it with cellular function.

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Zach Bjornson-Hooper, S.B.
Position: Ph.D. Candidate, Microbiology and Immunology
Education: S.B., Biology, Massachusetts Institute of Technology
Specialities: Virology, Genomics, Informatics
email:
Field: Microbiology & Immunology
Project(s):Long-term sequelae and immune response to Ebolavirus infection and analysis of high-dimensional flow cytometry data


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Robert Bruggner, B.S.
Position: Graduate Student.
Education: B.S. Computer Engineering & M.S. Biology, University of Notre Dame
Specialties: Informatics, genomics, web infrastructure technologies
email:
Field: Biomedical Informatics
Project(s): Analysis and infrastructure of high-dimensional flow cytometery data. Network analysis

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Shih-Yu Chen, Ph.D.
Position: Postdoctoral Fellow
Education: M.D., National Taiwan University; Ph.D., Neuroscience, University of California, Davis
Specialities: Cell Signaling
email:
Field: Translational research and Immunology
Project(s):Biomarker development in autoimmune disorders


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Tyler Burns, B.S.
Position: Ph.D. Candidate, IDP - Cancer Biology
Education: B.A., Human Biology, Stanford University
Specialities: Murine models of cancer and brain development
email:
Field: Cell signaling and non-coding RNA within the context of cancer.


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Matthew Clutter, B.S.
Position: Graduate Student
Education: University of Illinois Urbana-Champaign: B.S. in Biochemistry
Specialties: FACS phosphoprotein analysis, T cell biology, allergy
email:
Field: Immunology
Project(s): Allergy/asthma profiling, primary mast cell signaling

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Kara L. Davis D.O.
Position: Fellow, Pediatric Hematology/Oncology
Education: BA Pennsylvania State University; Medical School: Philadelphia College of Osteopathic Medicine
Residency: Pediatrics, Thomas Jefferson University
Specialties: Pediatric Hematology/Oncology
email:
Project(s): Signaling in high risk childhood ALL; Neuroblastoma

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Wendy Fantl, Ph.D.
Position: Senior Scientist
email:
Field: Microbiology and Immunology

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Harris Fienberg
Position: Graduate Student
Education: Stanford University: B.S. Biological Sciences, BA English; University of Basel: M.Sc. Zoology
Specialties: FACS phosphoprotein analysis, high throughput screening, new technique development
email:
Field: Microbiology and Immunology
Project(s): Leukemia primary cell profiling, mast cell signaling

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Rachel Finck
Position: Data Analyst
Education: B.S., Physics, B.S., Mathematics, Massachusetts Institute of Technology
Specialities: Methods for quality control and analysis of mass cytometry data
email:
Field: Data Analysis
Project(s): Mass cytometry data normalization, computational deconvolution of barcoded data, high-throughput dose response analysis


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Gabi Fragiadakis, B.A.
Position: Ph.D. Candidate, Microbiology and Immunology
Education: B.A., Molecular and Cellular Biology, University of California, Berkeley
email:

Gabi is interested in combining computational and experimental approaches to study biology. Specifically, she would like to bring together high dimensional flow cytometry and methods of data visualization, statistical analysis, and algorithm development to map out the healthy human immune system. This includes profiling both phenotypic surface expression and signaling networks. Gabi hopes to develop a framework for the healthy immune system to use as a reference for altered immunity and disease.


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Brice Gaudillière, Ph.D., M.D.
Position: Postdoctoral Fellow
Education: B.S., Engineering, Ecole Polytechnique; Ph.D., Neurobiology, Harvard Medical School; M.D., Harvard-MIT division of Health Sciences and Technology
Specialities: Anesthesiology
email:
Field: Anesthesiology, Perioperative Immunity
Project(s):using single cell mass cytometry to answer two questions: (1) how a patient's preoperative immune profile correlates with surgical recovery, and (2) how common anesthetic drugs (such as narcotics, sedatives or local anesthetics) modulate the immune response to surgical trauma.


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Yury Goltsev. Ph.D.
Position: Research Scientist
Education: M.Sc. Moscow Institute of Physics and Technology, Biophysics Ph.D. 2003 Weizmann Institute of Science, Molecular Immunology
Specialties: Signaling to programmed cell death from TNF/NGF receptor family. Evolution of Transcriptional Regulatory Networks
email:
Field: Immunology/Oncology
Project(s): Transcriptonal regulation of cell differentiation in normal and oncogenic hematopoiesis


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Howard Guss
Position: Executive Assistant
Education:
email:



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Matt Hale, Ph.D.
Position: Research Scientist
Education: Yale University: B.S. in Molecular Cellular and Developmental Biology, Stanford University: Ph.D. in Molecular Pharmacology
Specialties: FACS phosphoprotein analysis, dendritic cell immunology, Systemic Lupus Erythematosus
email:
Field: Immunology
Project(s): Understanding immune cell signal processing in autoimmunity

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Faye Y Hsu, Ph.D.
Position: Postdoctoral Fellow
Education: University of Southern California, Ph.D. in Biochemistry and Molecular Biology, M.S in Molecular Microbiology and Immunology
Specialties: Regulatory gene activity in lymphoma cells
email:
Field: Immunology and Cancer Biology
Project(s): Signalling mechanisms of tumor infiltrating T cells

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Astraea Jager
Position: Technician/Research Assistant
Specialties: B.S., Biological Sciences, San Jose State
email:

Astraea has been a technician/research assistant for about 4 years in the Nolan lab doing various immunology and molecular biology techniques. She has many years of experience in flow cytometry, working with the LSRII as well as one year of experience working with the CYTOF, including maintenance and operation. She also conjugates and titrates many of the metal-labeled antibodies used for CYTOF analysis.

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Gina Jager
Position: Technician
Specialties: FACS guru, antibody conjugation, cell line generation.  Garry's technician when he was a grad student in the Herzenberg laboratory.
email:

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Nikesh Kotecha, Ph.D.
Position: Senior Research Engineer
Education: Boston University: B.S. in Biomedical Engineering, Stanford University: PhD in Biomedical Informatics
Specialties: Signaling in leukemia, informatics, flow cytometry software, trans
email:
Field: Biomedical informatics, leukemia, software engineering
Project(s): Cell signaling in leukemia. Analysis and visualization methods for flow cytometry data, management and analysis infrastructure for translational research projects
More information: here

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Peter Krutzik, Ph.D.
Position: Research Associate
Education: University of California, Irvine: B.S. in Chemistry
Specialties: FACS phosphoprotein analysis, B cell immunology, organic chemistry, antibody conjugation
email:
Field: Immunology
Project(s): B cell development and cell signaling, multiparameter FACS drug screening

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Cynthia Lorang
Position: Research Technician
Education: B.S., Cellular, Molecular, and Developmental Biology, University of Washington
Specialities: Molecular Biology Techniques, Mass Spectrometry, Flow Cytometry, Proteomics
email:
Field: Immunology, Systems Biology, and Proteomics
Project(s):Developing a whole blood assay for clinical use to monitor the affect of cancer drug treatment by CYTOF analysis of immune cell signaling networks


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Rosalyn McCambridge
Position: Finance Specialist
Specialties: Background in small business management, Banking and Investment industies
email:



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Veronica Gonzalez Muñoz, Ph.D.
Position: Postdoctoral Fellow
Education: M.Sc., Molecular Biology, Stockholm University; Ph.D., Experimental Medicine, Karolinska Institutet; Postdoctoral Fellow Virology/Immunology, NIDDK/NIH
Specialities: T and NK cell biology, Viral host-pathogen interactions, HIV-1, HCV
email:
Field: Immunology
Project(s):Immune phenotyping and characterization of HIV-1 Elite controllers


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Dr. Garry Nolan, Ph.D.
Position: Professor
Field: Genetics
email:
Education:

  • Undergraduate: Cornell University: B.S. Biology (specialization: Genetics): Research in Rhizobium Genetics.

  • Graduate: Stanford University: Ph.D. Genetics. Laboratory of Leonard and Leonore Herzenberg. CD8 cloning; heritability of transcription states and FACS-Gal assay for in vivo measure of transcription.

  • Postdoctoral: MIT/Rockefeller University: Laboratory of David Baltimore. NF-kappa B, Bcl-3, BOSC23 transient retroviral producer system.

Specialties: Cloning/cDNA libraries, Flow Cytometry, Retroviral and Viral delivery systems, Transcription and Promoter Biology, HIV-1, Tat transporter proteins, Novel Apoptosis proteins and intracellular signaling relating to apoptosis, oncogenes and anti-oncogenes, NF-kappa B, NFAT, single cell measures of transcription, Carnivorous Plants, and making graduate students and postdocs feel guilty for not working nights and weekends.

Project(s): whatever it takes to get the job done!

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Above: Long-Term objectives!

Below: Tigger-2001!! 


Karen Sachs, Ph.D.
Position: Post Doc
Education: BSc Biology, Technion, Israeli Institute of Technology; PhD Bioengineering, Massachusetts Institute of Technology
Specialties: Probabilistic models of biological pathways
email:
Field: Computational models of signaling pathways in Immunology
Project(s): Models of B-cell signaling, Investigating differences in signaling across distinct samples and disease states

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Erica Savig, B.S., M. Arch.
Position: Ph.D. Candidate, IDP - Cancer Biology
Education: M. Arch. (Architecture), University of Pennsylvania B.S., Management Science & Engineering, Stanford University
Specialities: Data Visualization
email:
Field: Cancer Biology, Design Computation
Project(s):Applying architectural design computation methods to visualization and geometrically-driven analysis of flow cytometry data, while investigating local-tumor immune interactions in serous ovarian cancer.


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Erin Simonds, B.S.
Position: Graduate Student
Education: University of Connecticut, B.S. in Molecular & Cell Biology
Specialties: Phospho-specific flow cytometry, functional genomics, host defense, bioinformatics
email:
Field: Microbiology and Immunology
Project(s): Heterogeneous signaling subpopulations in childhood leukemias: Characterizing altered pathway biology, drug sensitivity and treatment escape mechanisms

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Matt Spitzer, B.S.
Position: Ph.D. Candidate, IDP - Immunology
email:

Matt Spitzer grew up in Manhattan Beach, CA and did his undergraduate work at Georgetown University, where he earned his bachelors degree in Biology with a minor in Economics. He performed his thesis research as a member of the Hoe lab, studying novel small-molecule therapeutics for Alzheimer's disease in a mouse model. He was also a member of the men's water polo team. Matt is the self-proclaimed mouse enthusiast of the Nolan Lab, and his thesis work focuses on using mass cytometry as a tool to unravel the mechanistic basis of tumor immunotherapy.


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Angelica Trejo, B.S.
Position: LSRA Lab Manager
Education: Stanford University: B.S. in Biology
Specialties: Sectioning, in situ hybridization, histochemistry, and confocal microscopy.
email:

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Eli Zunder, Ph.D.
Position: Post-Doctoral Fellow
Education: Dartmouth College: B.A. in Biochemistry, Cellular, and Molecular Biology, UCSF: Ph.D. in Biophysics
Specialities: FACS phosphoprotein analysis, kinase inhibition
email:
Field: Immunology, Cancer Biology
Project(s): Multiparameter FACS drug screening


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