Figure 5. Multiparameter staining: surface+intracellular staining
A) 1X106 PBMC (non-depleted) were treated with indicated cytokine (200 ng/ml, 15 min) and stained for CD16-cychrome (clone 3G8), CD19-PE (clone HIB19), and phospho-STAT6(Y641)-AX647 (clone 18) (as per protocol 3). Cells were gated for either CD16 or CD19 and displayed for phospho-STAT6. B) Cells were prepared as described above and stained with CD56-PE (clone B159), CD11B-cychrome (clone ICRF44) and phospho-STAT6(Y641)-AX647 (clone 18) (as per protocol 4).