Figure 6.
Multivariate analysis of complex populations
1X106 PBMC (non-depleted) were left untreated or treated with indicated
TNFa (200 ng/ml, 15 min) and stained for CD14-FITC (clone
MfP9), CD19-Cychrome (clone HIB19),
phospho-STAT3(Y705)-PE (clone 4), and phospho-STAT5(Y694)-AX647 (clone 47) (as per protocol 4). Multivariate
analysis was performed using clustering algorithms developed by Dr. Mario
Roederer in Flowjo 4.2