Figure 6. Multivariate analysis of complex populations

1X106  PBMC (non-depleted) were left untreated or treated with indicated TNFa (200 ng/ml, 15 min) and stained for CD14-FITC (clone MfP9), CD19-Cychrome (clone HIB19), phospho-STAT3(Y705)-PE (clone 4), and phospho-STAT5(Y694)-AX647  (clone 47) (as per protocol 4). Multivariate analysis was performed using clustering algorithms developed by Dr. Mario Roederer in Flowjo 4.2