Figure 3 Formaldehyde maintains scatter properties of permeabilized cells. Murine splenocytes were treated with red blood cell lysis buffer then placed in RPMI supplemented with 10% FBS for 1 hour. The cells were then left untreated, permeabilized directly with methanol, or fixed with formaldehyde (PFA) and then permeabilized with various reagents. A gate was drawn around lymphocytes in the unfixed sample to serve as a reference for effects on scatter caused by fixation/permeabilization. Numbers indicate percentage of cells in the lymphocyte gate.