Figure 3 Formaldehyde maintains scatter
properties of permeabilized cells. Murine
splenocytes were treated with red blood cell lysis buffer then placed in RPMI
supplemented with 10% FBS for 1 hour. The cells were then left untreated,
permeabilized directly with methanol, or fixed with formaldehyde (PFA) and then
permeabilized with various reagents. A gate was drawn around lymphocytes in the
unfixed sample to serve as a reference for effects on scatter caused by
fixation/permeabilization. Numbers indicate percentage of cells in the
lymphocyte gate.