Molecular cloning and chromosomal localization to 17q21 of the human WNT3 gene.
|Title||Molecular cloning and chromosomal localization to 17q21 of the human WNT3 gene. |
|Publication Type||Journal Article |
|Year of Publication||1993 |
|Authors||Roelink H, Wang J, Black DM, Solomon E, Nusse R |
|Date Published||Sep |
|ISSN||0888-7543 (Print); 0888-7543 (Linking) |
|Abstract||In mouse mammary tumors, the Wnt-3 gene can be activated by proviral insertion. Here we report on the isolation of a human homolog, WNT3. A genomic clone was isolated by use of mouse Wnt-3 sequences as a probe, after which cDNA containing most of the protein-encoding domain of the human gene was obtained by PCR. Comparison between the deduced mouse and human WNT-3 protein sequences showed four changes in 333 amino acids. WNT3 is located on chromosome 17q21. The gene was not found to be amplified or rearranged in a collection of human breast tumors. |