The nucleotide sequence of the human int-1 mammary oncogene; evolutionary conservation of coding and non-coding sequences.

TitleThe nucleotide sequence of the human int-1 mammary oncogene; evolutionary conservation of coding and non-coding sequences.
Publication TypeJournal Article
Year of Publication1985
Authorsvan Ooyen A, Kwee V, Nusse R
JournalEMBO J
Volume4
Pagination2905–2909
Date PublishedNov
ISSN0261-4189 (Print); 0261-4189 (Linking)
AbstractThe mouse mammary tumor virus can induce mammary tumors in mice by proviral activation of an evolutionarily conserved cellular oncogene called int-1. Here we present the nucleotide sequence of the human homologue of int-1, and compare it with the mouse gene. Like the mouse gene, the human homologue contains a reading frame of 370 amino acids, with only four substitutions. The amino acid changes are all in the hydrophobic leader domain of the int-1 encoded protein, and do not significantly alter its hydropathic index. The conservation between the mouse and the human int-1 genes is not restricted to exons; extensive parts of the introns are also homologous. Thus, int-1 ranks among the most conserved genes known, a property shared with other oncogenes.