Localization of a gene for expression of mouse mammary tumor virus antigens in the GR/Mtv-2- mouse strain.
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Title | Localization of a gene for expression of mouse mammary tumor virus antigens in the GR/Mtv-2- mouse strain. |
Publication Type | Journal Article |
Year of Publication | 1980 |
Authors | Nusse R, de Moes J, Hilkens J, van Nie R |
Journal | J Exp Med |
Volume | 152 |
Pagination | 712–719 |
Date Published | Sep |
ISSN | 0022-1007 (Print); 0022-1007 (Linking) |
Abstract | The GR/Mtv-2- mouse strain is congenic to the GR strain but lacks the Mtv-2 gene for high amounts of mouse mammary tumor virus (MMTV) virion particles in the milk and early mammary tumors. With a sensitive competition radioimmunoassay for individual viral proteins of MMTV, substantial amounts of the gag proteins p27 and p10 could still be detected in extracts of the mammary glands of GR/Mtv-2- mice, but essentially no viral envelope antigens. The genetic transmission of the MMTV gag expression in the GR/Mtv-2- strain was investigated. In a cross with the virus-negative BALB/c strain, the MMTV p27 expression behaved as a dominant feature. Double backcross analysis proved that the p27 expression was governed by a single gene located on chromosome 11, cloe to the Es-3 locus. The gene was thereby not allelic to any of the previously described MMTV induction genes, Mtv-1 and Mtv-2, and is therefore called Mtv-3. It is concluded that the total MMTV expression in the GR strain is under control of two separate loci, Mtv-2 on chromosome 18, inducing high levels of complete virus particles and also early mammary tumors; and Mtv-3 on chromosome 11, coding for partial MMTV expression. |