Tissue repair in the mouse liver following acute carbon tetrachloride depends on injury-induced Wnt/β-catenin signaling.

TitleTissue repair in the mouse liver following acute carbon tetrachloride depends on injury-induced Wnt/β-catenin signaling.
Publication TypeJournal Article
Year of Publication2019
AuthorsZhao L, Jin Y, Donahue K, Tsui M, Fish M, Logan CY, Wang B, Nusse R
JournalHepatology (Baltimore, Md.)
Date Published2019 Feb 14
ISSN0270-9139
AbstractIn the liver, Wnt/β-catenin signaling is involved in regulating zonation and hepatocyte proliferation during homeostasis. We have examined Wnt gene expression and signaling after injury and we show by in situ hybridization that Wnts are activated by acute carbon tetrachloride (CCl ) toxicity. Following injury, peri-injury hepatocytes become Wnt-responsive, expressing the Wnt target gene Axin2. Lineage tracing of peri-injury Axin2+ hepatocytes shows that during recovery, the injured parenchyma becomes repopulated and repaired by Axin2+ descendants. Using single cell RNA sequencing (scRNA-seq), we show that endothelial cells are the major source of Wnts following acute CCl toxicity. Induced loss of β-catenin in peri-injury hepatocytes results in delayed repair and ultimately to injury-induced lethality, while loss of Wnt production from endothelial cells leads to a delay in the proliferative response after injury. CONCLUSION: Our findings highlight the importance of the Wnt/β-catenin signaling pathway in restoring tissue integrity following acute liver toxicity and establishes a role of endothelial cells as an important Wnt-producing regulator of liver tissue repair following localized liver injury. This article is protected by copyright. All rights reserved.
URLhttps://doi.org/10.1002/hep.30563
DOI10.1002/hep.30563
Short TitleHepatology