Anaerobic activation of toluene and o-xylene by addition to fumarate in denitrifying strain T.

TitleAnaerobic activation of toluene and o-xylene by addition to fumarate in denitrifying strain T.
Publication TypeJournal Article
Year of Publication1997
AuthorsBeller, HR, Spormann AM
JournalJournal of bacteriology
Volume179
Issue3
Pagination670-6
Date Published1997 Feb
ISSN0021-9193
KeywordsAnaerobiosis, Benzylidene Compounds, Biodegradation, Environmental, Cell Membrane Permeability, Fumarates, Gram-Negative Aerobic Bacteria, Kinetics, Mass Spectrometry, Succinates, Toluene, Xylenes
AbstractAnaerobic assays conducted with strain T, a denitrifying bacterium capable of mineralizing toluene to carbon dioxide, demonstrated that toluene-grown, permeabilized cells catalyzed the addition of toluene to fumarate to form benzylsuccinate. This reaction was not dependent on the presence of coenzyme A (CoA) or ATP. In the presence of CoA, formation of E-phenylitaconate from benzylsuccinate was also observed. Kinetic studies demonstrated that the specific rate of benzylsuccinate formation from toluene and fumarate in assays with permeabilized cells was >30% of the specific rate of toluene consumption in whole-cell suspensions with nitrate; this observation suggests that benzylsuccinate formation may be the first reaction in anaerobic toluene degradation by strain T. Use of deuterium-labeled toluene and gas chromatography-mass spectrometry indicated that the H atom abstracted from the toluene methyl group during addition to fumarate was retained in the succinyl moiety of benzylsuccinate. In this study, no evidence was found to support previously proposed reactions of toluene with acetyl-CoA or succinyl-CoA. Toluene-grown, permeabilized cells of strain T also catalyzed the addition of o-xylene to fumarate to form (2-methylbenzyl)succinate. o-Xylene is not a growth substrate for strain T, and its transformation was probably cometabolic. With the exception of specific reaction rates, the observed characteristics of the toluene-fumarate addition reaction (i.e., retention of a methyl H atom and independence from CoA and ATP) also apply to the o-xylene-fumarate addition reaction. Thus, addition to fumarate may be a biochemical strategy to anaerobically activate a range of methylbenzenes.
Alternate JournalJ. Bacteriol.
0 November 24, 2010