Molecular identification of the catabolic vinyl chloride reductase from Dehalococcoides sp. strain VS and its environmental distribution.

TitleMolecular identification of the catabolic vinyl chloride reductase from Dehalococcoides sp. strain VS and its environmental distribution.
Publication TypeJournal Article
Year of Publication2004
AuthorsMüller, JA, Rosner BM, Von Abendroth G, Meshulam-Simon G, McCarty PL, Spormann AM
JournalApplied and environmental microbiology
Volume70
Issue8
Pagination4880-8
Date Published2004 Aug
ISSN0099-2240
KeywordsAmino Acid Sequence, Anaerobiosis, Biodegradation, Environmental, Chloroflexi, Cloning, Molecular, Computational Biology, Molecular Sequence Data, Oxidoreductases, Sequence Alignment, Sequence Analysis, DNA, Vinyl Chloride, Water Pollution, Chemical
AbstractReductive dehalogenation of vinyl chloride (VC) to ethene is the key step in complete anaerobic degradation of chlorinated ethenes. VC-reductive dehalogenase was partially purified from a highly enriched culture of the VC-respiring Dehalococcoides sp. strain VS. The enzyme reduced VC and all dichloroethene (DCE) isomers, but not tetrachloroethene (PCE) or trichloroethene (TCE), at high rates. By using reversed genetics, the corresponding gene (vcrA) was isolated and characterized. Based on the predicted amino acid sequence, VC reductase is a novel member of the family of corrinoid/iron-sulfur cluster containing reductive dehalogenases. The vcrA gene was found to be cotranscribed with vcrB, encoding a small hydrophobic protein presumably acting as membrane anchor for VC reductase, and vcrC, encoding a protein with similarity to transcriptional regulators of the NosR/NirI family. The vcrAB genes were subsequently found to be present and expressed in other cultures containing VC-respiring Dehalococcoides organisms and could be detected in water samples from a field site contaminated with chlorinated ethenes. Therefore, the vcrA gene identified here may be a useful molecular target for evaluating, predicting, and monitoring in situ reductive VC dehalogenation.
Alternate JournalAppl. Environ. Microbiol.
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