Pb(II) distributions at biofilm-metal oxide interfaces.

TitlePb(II) distributions at biofilm-metal oxide interfaces.
Publication TypeJournal Article
Year of Publication2001
AuthorsTempleton, AS, Trainor TP, Traina SJ, Spormann AM, Brown GE
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue21
Pagination11897-902
Date Published2001 Oct 9
ISSN0027-8424
KeywordsAluminum Oxide, Biofilms, Burkholderia cepacia, Cations, Divalent, Ferrous Compounds, Lead, Metals
AbstractThe distribution of aqueous Pb(II) sorbed at the interface between Burkholderia cepacia biofilms and hematite (alpha-Fe(2)O(3)) or corundum (alpha-Al(2)O(3)) surfaces has been probed by using an application of the long-period x-ray standing wave technique. Attached bacteria and adsorbed organic matter may interfere with sorption processes on metal oxide surfaces by changing the characteristics of the electrical double layer at the solid-solution interface, blocking surface sites, or providing a variety of new sites for metal binding. In this work, Pb L(alpha) fluorescence yield profiles for samples equilibrated with 10(-7) to 10(-3.8) M Pb(II) were measured and modeled to determine quantitatively the partitioning of Pb(II) at the biofilm-metal oxide interface. Our data show that the reactive sites on the metal oxide surfaces were not passivated by the formation of a monolayer biofilm. Instead, high-energy surface sites on the metal oxides form the dominant sink for Pb(II) at submicromolar concentrations, following the trend alpha-Fe(2)O(3) (0001) > alpha-Al(2)O(3) (1102) > alpha-Al(2)O(3) (0001), despite the greater site density within the overlying biofilms. At [Pb] > 10(-6) M, significant Pb uptake by the biofilms was observed.
Alternate JournalProc. Natl. Acad. Sci. U.S.A.
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