In vitro studies on reductive vinyl chloride dehalogenation by an anaerobic mixed culture.

TitleIn vitro studies on reductive vinyl chloride dehalogenation by an anaerobic mixed culture.
Publication TypeJournal Article
Year of Publication1997
AuthorsRosner, BM, McCarty PL, Spormann AM
JournalApplied and environmental microbiology
Volume63
Issue11
Pagination4139-44
Date Published1997 Nov
ISSN0099-2240
AbstractReductive dehalogenation of vinyl chloride (VC) was studied in an anaerobic mixed bacterial culture. In growth experiments, ethene formation from VC increased exponentially at a rate of about 0.019 h(sup-1). Reductive VC dehalogenation was measured in vitro by using cell extracts of the mixed culture. The apparent K(infm) for VC was determined to be about 76 (mu)M; the V(infmax) was about 28 nmol (middot) min(sup-1) (middot) mg of protein(sup-1). The VC-dehalogenating activity was membrane associated. Propyl iodide had an inhibitory effect on the VC-dehalogenating activity in the in vitro assay. However, this inhibition could not be reversed by illumination. Cell extracts also catalyzed the reductive dehalogenation of cis-1,2-dichloroethene (cis-DCE) and, at a lower rate, of trichloroethene (TCE). Tetrachloroethene (PCE) was not transformed. The results indicate that the reductive dehalogenation of VC and cis-DCE described here is different from previously reported reductive dehalogenation of PCE and TCE.
Alternate JournalAppl. Environ. Microbiol.
0 November 24, 2010