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    Stearns Lab Publications

    Vladar E.K., Stratton M.B., Saal M.L., Salazar-De Simone G, Wang X, Wolgemuth D, Stearns T, Axelrod J.D. (2018) Cyclin-dependent kinase control of motile ciliogenesis. Elife 7:e36375. (PDF)

    Mirvis M., Stearns T. and Nelson W.J. (2018) Cilium structure, assembly, and disassembly regulated by the cytoskeleton. Biochem J. 475:2329-2353. (PDF)

    Wang J.T. and Stearns T. (2018) The ABCs of centriole architecture: the form and function of triplet microtubules. CSHSQB 82:145-155. (PDF)

    Stratton M. and Stearns T. (2017) Mitosis sans mitosis: the mitotic oscillator in differentiation. Dev Cell 43:395-6. (PDF)

    Wang J.T., Kong, D., Hoerner C.R., Loncarek J., and Stearns T. (2017) Centriole triplet microtubules are required for stable centriole formation and inheritance in human cells. Elife 6:e29061. (PDF)

    Hekmat-Scafe D., Brownell S., Chandler P.S., Malladi S., Imam J., Singla V., Bradon N., Cyert M., and Stearns T. (2017) Using yeast to determine the functional consequences of mutations in the human p53 tumor suppressor gene: An introductory course-based undergraduate research experience in molecular and cell biology. Biochemistry and Molecular Biology Education. 45:161-178.

    Schatten G. and Stearns T. (2015) Sperm centrosomes: kiss your asterless goodbye, for fertility's sake. Curr. Biol.25:R1178-81. (PDF)

    Van de Mark D., Kong D., Loncarek J., and Stearns T. (2015) MDM1 is a microtubule-binding protein that negatively regulates centriole duplication. Mol Biol Cell 26:3788-802. (PDF)

    Turk E., Wills A.A., Kwon T., Sedzinski J., Wallingford J.B. and Stearns T. (2015) Zeta-Tubulin is a member of a conserved tubulin module and is a component of the centriolar basal foot in multiciliated cells. Curr. Biol. 25:2177-2183. (PDF, Supplement)

    Firat-Karalar E.N. and Stearns T. (2015) Probing mammalian centrosome structure using BioID proximity-dependent biotinylation. Methods in Cell Biology 129:153-170. (PDF)

    Stearns T. (2015) Centrioles, in absentia. Science 348:1091-92. (PDF)

    Brownell S.E., Hekmat-Scafe D.S., Singla V., Seawell P.C., Imam J.F.C., Eddy S.L., Stearns T. and Cyert M.S. (2015) A high-enrollment course-based undergraduate research experience improves student conceptions of scientific thinking and ability to interpret data. CBE Life Sci Educ 14:1-14. (PDF)

    Vladar E.K., Lee Y.L., Stearns T., and Axelrod J.D. (2015) Observing planar cell polarity in multiciliated mouse airway epithelial cells. Methods in Cilia & Flagella 1:37-54. (PDF)

    Firat-Karalar E.N., Sante J., Elliott S., and Stearns T. (2014) Proteomic analysis of mammalian sperm cells identifies new components of the centrosome. J Cell Sci 127:4128-4133. (PDF)

    Lee YL, Sante J, Comerci CJ, Cyge B, Menezes LF, Li FQ, Germino GG, Moerner WE, Takemaru K, Stearns T. (2014) Cby1 promotes Ahi1 recruitment to a ring-shaped domain at the centriole-cilium interface and facilitates proper cilium formation and function. Mol Biol Cell 25:2919-2933. (PDF)

    Firat-Karalar E.N. and Stearns T. (2014) The centriole duplication cycle. Phil. Trans. R. Soc. B 369:20130460. (PDF)

    Stearns T. (2014) Journey to the center of the centrosome. Dev Cell 28:603-604. (PDF)

    Lee J.Y., Hong W-J., Majeti R., and Stearns T. (2014) Centrosome-kinase fusions promote oncogenic signaling and disrupt centrosome function in myeloproliferative neooplasms. PLoS ONE 9:e92641. (PDF)

    Firat-Karalar E.N., Rauniyar N., Yates J.R., and Stearns T. (2014) Proximity interactions among centrosome components identify regulators of centriole duplication. Curr. Biol. 24:664-670. (PDF, Supplement)

    Hoerner C. and Stearns T. (2013) Remembrance of cilia past. Cell 155:271-273. (PDF)

    Tang Z., Lin M.G., Stowe T.R., Chen S., Zhu M., Stearns T., Franco B., and Zhong Q. (2013) Autophagy promotes primary ciliogenesis by removing OFD1 from centriolar satellites. Nature 502:254-257. (PDF)

    Tan F.E., Vladar E.K., Ma L., Fuentealba L.C., Hoh R., Espinoza F.H., Axelrod J.D., Alvarez-Buylla A., Stearns T., Kintner C., Krasnow M.A. (2013) Myb promotes centriole amplification and later steps of the multiciliogenesis program. Development 140:4277-4286. (PDF)

    Lee J.Y. and Stearns T. (2013) FOP is a centriolar satellite protein involved in ciliogenesis. PLoS ONE 8:e58589. (PDF)

    Akhmanova A. and Stearns T. (2013) Cell architecture: putting the building blocks together. Curr Opin Cell Biol. 25:3-5. (PDF)

    Schaub J.R. and Stearns T. (2013) The Rilp-like proteins Rilpl1 and Rilpl2 regulate ciliary membrane content. Mol Biol Cell. 24:453-64. (PDF)

    Hoh R.A., Stowe T.R., Turk E., and Stearns T. (2012) Transcriptional program of ciliated epithelial cells reveals new cilium and centrosome components and links to human disease. PLoS One 7:e52166. (PDF, Supplement)

    Mahjoub M.R. and Stearns T. (2012) Supernumerary centrosomes nucleate extra cilia and compromise primary cilium signaling. Curr. Biol. 22:1628-1634. (PDF, Supplement)

    Stowe T.R., Wilkinson C.J., Iqbal A., Stearns T. (2012) The centriolar satellite proteins Cep72 and Cep290 interact and are required for recruitment of BBS proteins to the cilium. Mol Biol Cell. 23:3322-3335. (PDF, Supplement)

    Lau L., Lee Y.L., Sahl S.J., Stearns T., and Moerner, W.E. (2012) STED microscopy with optimized labeling density reveals 9-fold arrangement of a centriole protein. Biophys J. 102:2926-2935. (PDF, Supplement)

    Temiyasathit S., Tang W.J., Leucht P., Anderson C.T., Monica S.D., Castillo A.B., Helms J.A., Stearns T., and Jacobs C.R. (2012) Mechanosensing by the primary cilium: deletion of Kif3A reduces bone formation due to loading. PLoS One 7:e33368. (PDF)

    Nigg E. and Stearns T. (2011) The centrosome cycle: centriole biogenesis, duplication and inherent asymmetries. Nature Cell Biol. 13:1154-60. (PDF)

    Park K.S., Martelotto L.G., Peifer M., Sos M.L., Karnezis A.N., Mahjoub M.R., Bernard K., Conklin J.F., Szczepny A., Yuan J., Guo R., Ospina B., Falzon J., Bennett S., Brown T.J., Markovic A., Devereux W.L., Ocasio C.A., Chen J.K., Stearns T., Thomas R.K., Dorsch M., Buonamici S., Watkins D.N., Peacock C.D., and Sage J. (2011) A crucial requirement for Hedgehog signaling in small cell lung cancer. Nature Med. 17:1504-1508. (PDF)

    Minear S., O'Donnell A.F., Ballew A., Giaever G., Nislow C., Stearns T. and Cyert M. (2011) Curcumin inhibits growth of Saccharomyces cerevisiae through iron chelation. Euk. Cell 10:1574-1581. (PDF)

    Hatch, E. and Stearns, T. (2011) The Life Cycle of Centrioles. Cold Spring Harbor Symp. Quant. Biol. 75:425-431. (PDF)

    Hatch, E.M., Kulukian, A., Holland, A.J., Cleveland, D.W., and Stearns, T. (2010) Cep152 interacts with Plk4 and is required for centriole duplication. J. Cell Biol. 191:721-729. (PDF, Commentary)

    Mahjoub, M.R., Xie, Z., and Stearns, T. (2010) Cep120 is asymmetrically localized to the daughter centriole and is essential for centriole assembly. J. Cell Biol. 191:331-346. (PDF)

    Stearns, T. (2009) Stem Cells: A Fateful Age Gap. Nature 461:891-892. (PDF)

    Tsou, M-F.B., Wang, W-J., George, K.A., Uryu, K., Stearns, T., and Jallepalli, P.V. (2009) Polo kinase and separase regulate the mitotic licensing of centriole duplication in human cells. Dev. Cell 17:344-354. (PDF, Supplement)

    Anderson, C.T., and Stearns T. (2009) Centriole Age Underlies Asynchronous Primary Cilium Growth in Mammalian Cells. Curr. Biol. 19:1498-1502. (PDF)

    Haren, L., Stearns, T. and Luders, J. (2009) Plk1-dependent recruitment of gamma-tubulin complexes to mitotic centrosomes involves multiple PCM components. PLoS ONE. 4:e5976. (PDF)

    Jaspersen, S.L. and Stearns, T. (2008) Exploring the pole: an EMBO conference on centrosomes and spindle pole bodies. Nature Cell Biol. 10:1375-1378. (PDF)

    Anderson, C.T., Castillo, A.B., Brugmann, S.A., Helms, J.A., Jacobs, C.R., and Stearns, T. (2008) Primary cilia: cellular sensors for the skeleton. Anat. Rec. 291:1074-1078. (PDF)

    Malone, A.M.D., Anderson, C.T., Tummala, P., Kwon, R.Y., Johnston, T.R., Stearns, T. and Jacobs, C.R. (2007) Primary cilia mediate mechanosensing in bone cells by a calcium-independent mechanism. PNAS 104:13325-13330. (PDF)

    Vladar, E.K. and Stearns, T. (2007) Molecular characterization of centriole assembly in ciliated epithelial cells. J Cell Biol. 178:31-42. (PDF, Supplementary data)

    Luders, J. and Stearns, T. (2007) Microtubule-organizing centres: a re-evaluation. Nature Rev Mol Cell Biol. 8:161-167. (PDF)

    Tsou, M-F.B. and Stearns, T. (2006) Mechanism limiting centrosome duplication to once per cell cycle. Nature 442:947-951. (PDF, Supplementary info, News and Views)

    Luders, J., Patel, U.K., and Stearns, T. (2006) GCP-WD is a gamma-tubulin targeting factor required for centrosomal and chromatin-mediated microtubule nucleation. Nature Cell Biol. 8:137-147. (PDF)

    Tsou, M-F.B. and Stearns, T. (2006) Controlling centrosome number: licenses and blocks. Curr. Opin. Cell Biol. 18:74-78. (PDF)

    Aldaz, H., Rice, L.M., Stearns, T., and Agard, D.A. (2005) Insights into microtubule nucleation from the crystal structure of human gamma-tubulin. Nature 435:523-527. (PDF)

    Wong, C. and Stearns, T. (2005) Mammalian cells lack checkpoints for tetraploidy, aberrant centrosome number, and cytokinesis failure. BMC Cell Biol. 6:6. (PDF)

    Louie, R.K., Bahmanyar, S., Siemers, K.A., Votin, V., Chang, P., Stearns, T., Nelson, W.J., and Barth, A.I. (2004) Adenomatous polyposis coli and EB1 localize in close proximity of the mother centriole and EB1 is a functional component of centrosomes. J Cell Sci. 117:1117-1128. (PDF)

    Wong, C. and Stearns, T. (2003) Centrosome number is controlled by a centrosome-intrinsic block to reduplication. Nature Cell Biol. 5:539-544. (PDF)

    Wong, C. and Stearns, T. (2003) A SAS-sy centriole in the cell cycle. Curr. Biol. 13:R351-352. (PDF)

    Chang, P., Giddings, T. H., Winey, M., and Stearns, T. (2003) Epsilon-tubulin is required for centriole duplication and microtubule organization. Nature Cell Biol. 5:71-76. (PDF)

    Patel U., Stearns T. (2002) Quick Guide: Gamma-Tubulin. Curr Biol. 2002 12:R408. (PDF)

    Click, E., Stearns, T., and Botstein, D. (2002) Systematic structure-function analysis of the small GTPase Arf1 in yeast. Mol. Biol. Cell 13:1652-1664. (PDF).

    Murphy, S.M., Preble, A.M., Patel, U.K., O'Connell, K.L., Dias, D.P., Moritz, M., Agard, D., Stults, J.T., and Stearns, T. (2001) GCP5 and GCP6: Two new members of the human gamma-tubulin complex. Mol. Biol. Cell 12:3340-3352. (PDF).

    Stearns, T. (2001) Centrosome duplication: a centriolar pas de deux. Cell 105:417-420. (PDF)

    Demeter, J., Lee, S.E., Haber, J.E. and Stearns, T. (2000) The DNA damage checkpoint signal in yeast is nuclear-limited. Mol. Cell 6:487-492. (PDF)

    Chang, P. and Stearns, T. (2000) Delta-tubulin and epsilon-tubulin: two new human centrosomal tubulins reveal new aspects of centrosome structure and function. Nature Cell Biol. 2:30-35. (PDF)

    Freed, E., Lacey, K. R., Lyapina, S. A., Huie, P., Deshaies, R. J., Stearns, T., and Jackson, P. (1999) The SKP1 and CUL1 ubiquitin ligase components localize to the centrosome and regulate the centrosome duplication cycle. Genes Dev. 13:2242-2257. (PDF)

    Jeng, R. and Stearns, T. (1999) Gamma-Tubulin complexes: size does matter. Trends Cell Biol. 9:339-342. (PDF)

    Urbani, L., and Stearns, T. (1999) The centrosome: a primer. Curr. Biol. 9:R315-R317. (PDF)

    Lacey, K. R., Jackson, P. K., and Stearns, T. (1999) Cyclin-dependent kinase control of centrosome duplication. Proc. Natl. Acad. Sci. 96:2817-2822. (PDF)

    Feierbach, B., Nogales, E., Downing, K. H., Stearns, T. (1999) Alf1p, a CLIP-170 domain-containing protein, is functionally and physically associated with a-tubulin. J. Cell Biol. 144:113-124. (PDF)

    Carminati, J. and Stearns, T. (1998) Cytoskeletal dynamics in yeast. Meth. Cell Biol. 58:87-105.

    Manandhar, G., Sutovsky, P., Joshi, H.C., Stearns, T., Schatten, G. (1998) Centrosome reduction during mouse spermiogenesis. Dev. Biol. 203:424-434.

    Murphy, S. M., Urbani, L., and Stearns, T. (1998) The mammalian gamma-tubulin complex contains homologs of the yeast spindle pole body components Spc97p and Spc98p. J. Cell Biol. 141:663-674. (PDF)

    Tucker, J. B., Mogensen, M. M., Henderson, C. G., Doxsey, S. J., Wright, M., and Stearns, T. (1998) Nucleation and capture of large cell-associated microtubule arrays that are not located near centrosomes in certain cochlear epithelial cells. J. Anat. 192:119-130. (PDF)

    Cho R.J., Fromont-Racine M., Wodicka L., Feierbach B., Stearns T., Legrain P., Lockhart D.J., Davis, R.W. (1988) Parallel analysis of genetic selections using whole genome oligonucleotide arrays. Proc. Natl. Acad. Sci. 1998 95:3752-3757.

    Leask, A. and Stearns, T. (1998) Expression of amino- and carboxy-terminal gamma- and alpha-tubulin mutants in cultured epithelial cells. J. Biol. Chem. 273:2661-2668. (PDF)

    Stearns, T. and Winey, M. (1997) The cell center at 100. Cell 91:303-309. (PDF)

    Adams, A., Gottschling, D. E., Kaiser, C. A., and Stearns, T. (1997) "Methods in Yeast Genetics". Cold Spring Harbor Press.  178 pp.

    Marschall, L. G. and Stearns, T. (1997) Anatomy of an organizing center. Curr. Biol. 7:R754-R756.

    Stearns, T. (1997) Motoring to the finish: kinesin and dynein work together to orient the yeast mitotic spindle. J. Cell Biol. 138:957-960. (PDF)

    Tian, G., Lewis, S.A., Feierbach, B., Stearns, T., Rommelaere, H., Ampe, C., Cowan, N.J. (1997) Tubulin subunits exist in an activated conformational state generated and maintained by protein cofactors.  J. Cell Biol. 138:821-832.

    Carminati, J. and Stearns, T. (1997) Microtubules orient the yeast spindle through dynein-dependent interactions with the cell cortex.  J. Cell Biol. 138:629-641. (video data) (PDF)

    Leask, A., Obrietan, K.,  and Stearns, T. (1997) Synaptically-coupled central nervous system neurons lack centrosomal gamma-tubulin.  Neuroscience Letters 229:17-20. (PDF)

    Botstein, D., Amberg, D., Huffaker, T., Mulholland, J., Adams, A., Drubin, D., Stearns, T. (1997)  The yeast cytoskeleton, in "The Molecular and Cellular Biology of the the Yeast Saccharomyces cerevisiae: Cell cycle and cell biology. Vol. 3."  Cold Spring Harbor Press. pg. 1-90.

    Vogel, J. M., Stearns, T., Rieder, C. L., Palazzo, R.E. (1997) Centrosomes isolated from Spisula solidissima oocytes contain rings and an unusual stoichiometric ratio of alpha/beta tubulin.  J. Cell Biol. 137:193-202.

    Mogensen, M. M., Mackie, J. B., Doxsey, S. J., Stearns, T., and Tucker, J. B. (1997)  Centrosomal deployment of gamma-tubulin and pericentrin: Evidence for a microtubule-nucleating domain and a minus-end docking domain in certain mouse epithelial cells.  Cell Motil. Cytoskel. 36:276-290. (PDF)

    Marschall, L. G., Jeng, R. L., Mulholland, J. and Stearns, T. (1996)  Analysis of Tub4p, a yeast gamma-tubulin-like protein: implications for microtubule-organizing center function.  J. Cell Biol. 134:443-454. (PDF)

    Murphy, S. M., and Stearns, T. (1996)  Microtubule nucleation takes shape. Curr. Biol. 6:642-644.

    Stearns, T. (1995) The form and the substance. Nature Medicine 1:19-20.

    Stearns, T. (1995) Green fluorescent protein: the green revolution.  Curr. Biol. 5:262-264. (PDF)

    Stearns, T. and Kirschner, M. (1994)  In vitro reconstitution of centrosome assembly and function: the central role of gamma-tubulin.  Cell 76:623-638. (PDF)

    Dunn, B., Stearns, T., and Botstein, D.  (1993)  Specificity domains distinguish the ras-related GTPases SEC4 and YPT1.  Nature 362:563-565. (PDF)

    Stearns, T. (1992) Cellular structures. Science 257:1422 (PDF)

    Stearns, T., Evans, L., and Kirschner, M. (1991)  Gamma-tubulin is a highly conserved component of the centrosome. Cell 65:825-836.(PDF)