Primary Reference Citations from 1999-2000


Below are a list of links to primary references, both abstracts and articles, pertaining to adenovirus. The partial citations, brief article synopses, and links to these articles are all provided below.

Many of the following articles are from 2000. Not surprisingly, a lot of research is being done on adenoviruses because of their utility in gene therapy. A search on Pubmed for Adenoviruses during the previous month yielded more than 100 results. A similar search on Ovid during the previous year gave more than 300 hits. Though the following articles do not give a comprehensive picture of the frontiers of adenovirus research, they demonstrate the diversity of adenovirus research that is currently being conducted. As John A Wheeler astutely observed, "We live on an island of knowledge surrounded by a sea of ignorance. As our island of knowledge grows, so does the shore of our ignorance." Fortunately, however, adenoviruses are an island that is growing exponentially and the shores are being explored at a similarly rapid pace, as the following articles demonstrate.


  1. Amalfitano A. "Next-generation adenoviral vectors: new and improved." Gene Ther.

October, 1999. 6(10): 1643-5.


2. Balakireva L. et al. "Role of Cellular Lipids in Adenovirus Entry." J. of Neurochemistry. 1999. 73(S): S67.

An exploration of the role of cellular lipids in viral entry into animal cells. Adenoviruses are not enveloped, and despite their widespread use in gene therapy, this mechanism has yet to be elucidated. The study found that the virus, outside the cell, was able to bind to cellular phosphatidylchoilne, thus suggesting a likely method for the viral attachment to the cell surface.


3.Campbell I. et al. "Adenoviral transfer of xenogeneic MHC class 1 gene results in loss of tumorigenicity and inhibition of tumor growth." Cancer Gene Ther. January, 2000. 7(1): 37-44.

After promising tests in rats, it is possible that adenoviral-mediated transfer of xenogeneic MHC class I gene may be a possible method for treating cancer. This is another area of gene therapy research.


4. Couroucli X.I. et al. "Detection of Microorganisms in the Tracheal Aspirates of Preterm Infants by Polymerase Chain Reaction: Association of Adenovirus Infection with Bronchopulmonary Dysplasia." Pediatric Research. February, 2000. 47(2): 225.

In infants with bronchopulmonary dysplasia (BPD), a higher frequency of the adenovirus genome was found. These adenovirus infections were acquired prenatally and may increase the risk of a newborn developing BPD.

5. Danthinne X. "New vectors for the construction of double recombinant adenovirus." J Virol Methods. August, 1999. 81(1-2): 11-20.

A discussion of how plasmids can be used to construct recombinant adenoviral vectors.


6. Danthinne X. et al. "New tools for the generation of E1- and/or E3-substituted adenoviral vectors." Gene Ther. January, 2000. 7(1): 80-7.

New vectors for creating recombinant adenoviruses have been created.


7. Engler H. "Enhanced Adenovirus-Mediated Gene Transfer to the Urothelium for Gene Therapy of Transitional Cell Carcinoma." The Journal of Urology. April, 1999. 161:4S: 120.

This article examinations the utility of treating bladder cancer with a p53 tumor suppressor gene via a recombinant adenovirus gene transfer. Because the bladder epithelium is not very permeable to the virus, some procedural modifications must be examined.


8. Ginsberg HS. "The life and times of adenoviruses." Adv Virus Res. 1999. 54: 1-13.

Background information on virion structure, genome, replication, and pathogenesis is discussed. This article also explores the importance of early viral genes for the full pathogenesis of Ad5 in mice.


9. Hall M.C. et al. "The growth inhibitory effect of p21 adenovirus on human bladder cancer cells." J Urol. March, 2000. 163(3): 1033-8.

p21 adenoviral gene transfer has demonstrated initial evidence that it may help to fight certain human bladder cancers. However, bladder cancers have shown a wide range of responsiveness to adenovirus "infection."


10. Hogg J.C. "The Adenovirus and Bronchopulmonary Dysplasia: An Association That Could Be Causal or Coincidental: Commentary on the article by Couroucli et a. On page 225." Pediatric Research. February, 2000. 47(2): 175.

Urges that the article by Couroucli that found a coralational relationship between bronchopulmonary dysplasia (BPD) and adenoviral infection must be challenged before a causal link can be drawn. Further laboratory examination is necessary before this conclusion can be made comfortably.


11. Kagawa S. et al. "A binary adenoviral vector system for expressing high levels of the proapoptotic gene bax." Gene Ther. January, 2000. 7(1): 75-9.

The bax gene is important in cellular apoptosis. This article reports a method for safely introducing this bax gene into the cell via adenovirus-mediated gene co-transfer. Previously, the development of an adenovirus vector for the bax gene has proven to be difficult.


12. Karpati G. et al. "Combination Therapy for Experimental Malignant Glioma Using Adenovirus-Mediated Transfer of p53 and Cytosine Deaminase Suicide Gene." Neurology. April 12, 1999. 52(6) S: A474-5.

In order to treat intracerebral gliomas in rats, both p53 and the suicide gene cytosine were used individually and in conjunction with each other. It was found that these recombinant adenovirus treatments were more effective in conjunction with each other as multiple injections.


13. Kochanek S. "High-capacity adenoviral vectors for gene transfer and somatic gene therapy." Hum Gene Ther. October 10, 1999. 10(15): 2451-9.

Discussion of safe and efficient adenovirus gene delivery technology. With this technology, gene transfer of many expression cassettes, large promoters, and genes in their normal genomic context, can all be conferred with one vector.


14. Lang F.F. "Adenovirus-mediated p53 Gene Therapy for Human Gliomas." Neurosurgery. November, 1999. 45(5): 1093.

The use of recombinant adenovirus with the p53 gene may be a strong treatment for human gliomas. In the article, possible benefits and complications are comprehensively delineated.


15. Mehrara B.J. "Adenovirus-Mediated Gene Therapy of Osteoblasts in Vitro and In Vivo." Journal of Bone & Mineral Research. August, 1999. 14(8): 1290-1301.

Adenovirus vectors containing the human TGF-[beta]1 can be used as gene therapy to help reduce the number and severity of fracture healing complications.


16. Motoi F. et al. "Effective gene therapy for pancreatic cancer by cytokines mediated by restricted replication-competent adenoviruses." Hum Gen Ther. January 20, 2000. 11(2): 223-35.

Sometimes, pancreatic cancer is thought to be caused by genetic abnormalities in either oncogenes or tumor suppressor genes. One of these genes is p53. Research suggests that adenovirus gene therapy may be an effective tool for treating these pancreatic cancers caused by p53 deficiencies.


17. Parks R.J. et al. "Adenoviral vectors: prospects for gene delivery to the central nervous system." Gene Ther. Aug, 1999. 6(8): 1349-50.


18. Ruzindana-Umunyana A. et al. "Adenovirus Endopeptidase Hydrolyses Human Squamous Cell Carcinoma Antigens in Vitro but not ex Vivo." Virology. March 1, 2000. 1: 141-6.

An examination of the relationship between group C adenovirus infection and expression of serpins SCCA1 and SCCA2 in the epithelium of the infected airway.


19. Senior K. "Link found between adenovirus infection and left-ventricular failure." The Lancet. March 20, 1999. 353(9157): 988.

Though a causal relationship has not definitely been established, a correlation has been found between adenovirus infection and increased likelihood of having ventricular dysfunction and sudden death.


20. Simons M. et al. "Adenovirus-mediated gene transfer of inhibitors of apoptosis proteins delays apoptosis in cerebellar granule neurons." J of Neurochemistry. January, 1999. 72(1): 292-301.

Genes that help to inhibit apoptosis (IAP) were introduced into cerebellar granule neurons in order to prevent cell death. Though this adenoviral-mediated gene transfer helped to delay apoptosis, it failed to slow this process beyond 24 hours.


21. Takayanagi H. et al. "Suppression of arthritic bone destruction by adenovirus-mediated csk gene transfer to synoviocytes and osteoclasts." J of Clin Invest. July, 1999. 104(2): 137-46.

Adenovirus-mediated gene transfer has been used in rats to treat symptoms of rheumatoid arthritis. Large expressions of the Csk gene helped deter the bone-resorbing osteoclast activity.


22. Van Raaij M.J. et al. "A triple [beta]-spiral in the adenovirus fibre shaft reveals a new structural motif for a fibrous protein." Nature. October 28, 1999. 401(6756): 935-8.

New insight into the structure of the adenovirus. A triple [beta]-spiral fibrous fold is found in the shaft of a recombinant adenoviral protein.


23. Wang K. et al. "Regulation of Adenovirus Membrane Penetration by the Cytoplasmic Tail of Integrin beta5." J Virol. March 15, 2000. 74(6): 2731-9.

An experiment examining the role of the integrin beta5 subunit is determining the adenovirusí membrane permeabilization and gene delivery.


24. Zhang W. et al. "Interaction of the Adenovirus Iva2 Protein with Viral Packaging Sequences." J Virol. March 15, 2000. 74(6): 2687-93.

This experiment looks at the relationship between the adenovirus L1 52/55-kDa protein and the viral Iva2 protein in infected cells to which it binds.


25. Zhao J. et al. "P53 Enhanced the Antitumoral Efficacy of E1B 55-Kilodalton Mutant Adenovirus on Hepatocellular Carcinoma Cell Line." Gut. April, 1999. 44(4S): 87A.

An examination of the effects of p53 on the cytotoxic effects of dl1520. When Ad-p53 and dl1520 were both present, the cytotoxic effects on the hepatocellular carcinoma cells were increased.