Richard's work has focused on developing GMR nanosensor arrays that exhibit exceptionally high sensitivity with a broad linear dynamic range for multiplex in-vitro diagnostics and biomolecular kinetic analysis. By labeling antibodies with magnetic nanoparticle tags, his work has demonstrated the GMR nanosensors' unique ability to perform "matrix-insensitive" protein detection and its application to highly sensitive and multiplex tumor marker screening (Figure 1). More specifically, his work has focused on early diagnosis of cancer as well as monitoring patient response to chemotherapy. A second focus of his work has been to model protein-protein binding kinetics using high density GMR nanosensor arrays and a novel mathematical model (Figure 2). A third project which Richard has pioneered was an autoassembly immunoassay developed for rapid antibody cross-reactivity screening and for wash-free proteomic analyses. The application of this autoassembly immunoassay, in conjunction with a miniaturized magnetic nanosensor platform has also been developed for point-of-care diagnostics (POC) in a cost-effective and wash-free manner for molecular diagnostics in the developing world (Figure 3). His POC work has focused on the detection of HIV and Hepatitis C in remote field settings.
 

Figure 2 Figure 3

Figure 2

Figure 3

About Richard's research on Magnetic Nanosensors:

Figure 1

Figure 1

Publications:

15. R.S. Gaster, L. Xu, S.J. Han, R.J. Wilson, D.A. Hall, S.J. Osterfeld, H. Yu, S.X. Wang. "Quantification of Protein Interactions and Solution Transport Using High-Density GMR Sensor Arrays." Nature Nanotechnology. vol. 6, 2011, pp. 314-320.

Featured in Nature Nanotechnology, News and Views: “Biosensors: Magnets tackle kineticquestions” 6, 266-267 (2011)

14. R.S. Gaster, D. A. Hall, and S. X. Wang, "nanoLAB: An ultraportable, handheld diagnostic laboratory for global health," Lab on a Chip, vol. 11, 2011, pp. 950-956.

This article was one of the top ten accessed articles in February 2011

13. R.S. Gaster, D.A. Hall, S.X. Wang. "Auto-Assembly Protein Arrays for Analyzing Antibody Cross-Reactivity." in press. NanoLetters

Selected for July 2011 Cover Art

12. D. Hall, R.S. Gaster, S. Osterfeld, B. Murmann, and S. Wang, "GMR biosensor arrays: Correction techniques for reproducibility and enhanced sensitivity," Biosensors and Bioelectronics, vol. 25, May. 2010, pp. 2177-2181.

11. D. Hall, R.S. Gaster, T. Lin, S. Osterfeld, S. Han, B. Murmann, and S. Wang, "GMR biosensor arrays: A system perspective," Biosensors and Bioelectronics, vol. 25, May. 2010, pp. 2051-2057.

10. R.S. Gaster, D.A. Hall, C.H. Nielsen, S.J. Osterfeld, H. Yu, K.E. Mach, R.J. Wilson, B. Murmann, J.C. Liao, S.S. Gambhir, and S.X. Wang, "Matrix-insensitive protein assays push the limits of biosensors in medicine," Nature Medicine, vol. 15, Nov. 2009, pp. 1327-1332.

Featured in Nature, News and Views: "Analytical chemistry: The matrix neutralized" 461: 890-891

9. R.J. Wilson, W. Hu, C.W.P. Fu, A.L. Koh, R.S. Gaster, C.M. Earhart, A. Fu, S.C. Heilshorn, R. Sinclair, and S.X. Wang, "Formation and properties of magnetic chains for 100 nm nanoparticles used in separations of molecules and cells," Journal of Magnetism and Magnetic Materials, vol. 321, May. 2009, pp. 1452-1458.

8. S.J. Osterfeld, H. Yu, R.S. Gaster, S. Caramuta, L. Xu, S. Han, D.A. Hall, R.J. Wilson, S. Sun, R.L. White, R.W. Davis, N. Pourmand, and S.X. Wang, "Multiplex protein assays based on real-time magnetic nanotag sensing," Proceedings of the National Academy of Sciences, vol. 105, Dec. 2008, pp. 20637-20640.

7. J. Mai, R.S. Gaster, A. Wu, W. Gu, K. Mach, and J. Liao, "A microfluidic system for rapid bacterial pathogen detection," 2007 7th IEEE International Conference on Nanotechnology – IEEE-NANO 2007, Proceedings, 2007, pp. 1330-1334.

6. M. Chorny, I. Fishbein, I.S. Alferiev, O. Nyanguile, R. Gaster, and R.J. Levy, "Adenoviral Gene Vector Tethering to Nanoparticle Surfaces Results in Receptor-Independent Cell Entry and Increased Transgene Expression," Molecular Therapy, vol. 14, 2006, pp. 382-391.

5. I. Fishbein, I.S. Alferiev, O. Nyanguile, R. Gaster, J.M. Vohs, G.S. Wong, H. Felderman, I. Chen, H. Choi, R.L. Wilensky, and R.J. Levy, "Bisphosphonate-mediated gene vector delivery from the metal surfaces of stents," Proceedings of the National Academy of Sciences of the United States of America, vol. 103, Jan. 2006, pp. 159-164.

4. B. Azarbal, J. Tobis, W. Suh, V. Chan, C. Dao, and R. Gaster, "Association of interatrial shunts and migraine headaches: Impact of transcatheter closure," Journal of the American College of Cardiology, vol. 45, 2005, pp. 489-492.

3. I. Fishbein, I. Alferiev, R. Gaster, M. Chorny, O. Nyanguile, R. Levy. "Transduction Enhancement by the Adenovirus/Polymer Complexes Derivatized with Protein Transduction Domain Peptides." Molecular Therapy, 11 (1): S340-S341, 2005.

2. A. Chhabra, B. Azerbal, H. Anzai, M. Fishbein, C. Dao, V. Chan, R. Gaster, J. Moore, J. Tobis. "Stability of the Amplatzer septal occluder device: Importance of the atrial tissue rim." Journal of the American College of Cardiology, 43: A376-A377, 2004.

1. I. Fishbein, I. Alferiev, R. Gaster, R. L. Wilensky, R. J. Levy. "Stent-Based Vascular Gene Transfer by Hydrolysis-mediated Release of Adenoviral Vectors." Circulation, 110 (17): III- 617, 2004.

Book Chapters:

2. Richard S. Gaster, Drew A. Hall, and Shan X. Wang, Magneto-nanosensor diagnostic chips, book chapter in Point of Care Diagnostics on a Chip, Editors: Lee, Ham, and Westervelt, Springer, In Preparation , 2011

1. Drew A. Hall, Richard S. Gaster, and Shan X. Wang, GMR Biosensors, book chapter in Spin Transport and Magnetism in Electronic Systems, Editors: Evgeny Tsymbal and Igor Žutić, Taylor & Francis, In Press, 2009.