Dr.Angela Barth, Stanford University


The NH2-Terminus of beta-catenin contains a potential recognition site for

the serine/threonine kinase glycogen synthase kinase (GSK)-3beta. Mutation

of this site increases the stability of beta-catenin in the adenomatous

polyposis coli (APC) protein complex. DeltaGSK-beta-catenin accumulates in

microtubule-associated APC protein clusters at the tip of cell extensions.


Movie A: The dynamics of these clusters are visualized by time lapse

confocal microscopy of MDCK cells expressing a fusion of

DGSK-beta-catenin with green fluorescent protein (GFP).

56 Frames collected every 40 sec, total time of movie: 37 min

(For a 750 KB QuickTime movie of better quality, click here)


Movie B: Time lapse confocal microscopy after co-transfection of MDCK cells

with GFP-DGSK-beta-catenin and GFP-tubulin. The DGSK-beta-catenin cluster

are localized at the end of microtubule filaments.

94 Frames collected every 27 sec, total time of movie: 42 min.

(For a 2.6 MB QuickTime movie of better quiality, click here)

GFP-fusion constructs made by Eugenio L. de Hostos, Department of Biochemistry and Cell Biology, Rice University. Transfections

and imaging by Angela I. Barth, Department of Molecular Physiology, Stanford University School of Medicine, with the assistance of

Cynthia L Adams, Stephen J. Smith, and W. James Nelson, Stanford.

Laser confocal microscope designed and built by Stephen J. Smith (see Adams, Nelson, and Smith, 1996, J. Cell Biol. 135:


Additional movies can be found in Dr. Eugenio de Hostos' Movie Web Page

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