phiC31 integrase in liver  
Stanford University  
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Michele Calos

Michele Pamela Calos

Professor
Department of Genetics

Publications


Calos, M.P. (1978). DNA sequence for a low-level promoter of the lac repressor gene and an "up" promoter mutation. Nature 274, 762 -765.

Calos, M.P., Johnsrud, L., and Miller, J.H. (1978). DNA sequence at the integration sites of the insertion element IS1. Cell 13, 411 - 418.

Johnsrud, L., Calos, M.P., and Miller, J.H. (1978). The transposon Tn9 generates a 9 bp repeated sequence during integration. Cell 15, 1209 - 1219.

Calos, M.P., Johnsrud, L., and Miller, J.H. (1979). Genetic studies of the lac repressor. VIII. DNA sequence change resulting from an intragenic duplication. J. Mol. Biol. 126, 865 - 869.

Calos, M.P., Johnsrud, L., and Miller, J.H. (1979) A genetic and sequencing study of IS1 and Tn9 insertions in the Escherichia coli lac operon. Cold Spring Harbor Symp. Quant. Biol. 43, 1263 - 1268.

Calos, M.P. and Miller, J.H. (1980). DNA sequence alteration resulting from a mutation impairing promoter function in the lac repressor gene. Mol. Gen. Genet. 178, 225-227.

Miller, J.H., Calos, M.P., Galas, D.J., Hofer, M., Buchel, D., and Muller-Hill, B. (1980). Genetic analysis of transpositions in the lac region of E. coli. J. Mol. Bio. 144, 1 - 18.

Galas, D.J., Calos, M.P., and Miller, J.H. (1980). Sequence analysis of Tn9 insertions in the lacZ gene. J. Mol. Biol. 144. 19 – 41.

Calos, M.P. and Miller, J.H. (1980). Molecular consequences of deletion formation mediated by the transposon Tn9. Nature 285, 38 – 41.

Calos, M.P. and Miller, J.H. (1980). Transposable elements. Cell 20, 579 - 595.

Calos, M.P. and Miller, J.H. (1980). Molecular biology in the People’s Republic of China. Trends in Biochemical Sciences 5, III-IV.

Miller, J.H., Calos, M.P., and Galas, D.J. (1981) Genetic and sequencing studies of the specificity of transpositions into the lac region of E. coli. Cold Spring Harbor Symp. Quant. Biol. 45, 243-257.

Calos, M.P. and Miller, J.H. (1981). The DNA sequence change resulting from the IQ1 mutation, which greatly increases promoter strength. Mol. Gen. Genet. 183, 559-560.

Calos, M.P. and Miller, J.H. (1981). Genetic and sequence analysis of frameshift mutations induced by ICR-191. J. Mol. Biol. 153, 39-64.

Miller, J.H., Calos, M.P., Combepine, C., and Hofer, M. (1981). Appendix: Further correlations of the lacI genetic map with the DNA sequence. J. Mol. Biol. 153, 65-66.

Albertini, A., Hofer, M., Calos, M.P., and Miller, J.H. (1982). On the formation of spontaneous deletions: The importance of short sequence homologies in the generation of large deletions. Cell 29, 319-328.

Albertini, A.M., Hofer, M., Calos, M.P., Tlsty, T.D., and Miller, J.H. (1983). Analysis of spontaneous deletions and gene amplification in the lac region of E. coli. Cold Spring Harbor Symp. Quant. Biol. 47, 841-850.

Calos, M.P., Lebkowski, J.S., and Botchan, M.R. (1983). High mutation frequency in DNA transfected into mammalian cells. Proc. Natl. Acad. Sci USA 82, 3015-3019.

Calos, M.P. (1983). Book review of Mobile Genetic Elements, ed. J.A. Shapiro, Science 221, 1366-1367.

Clancy, S., Mann, C., Davis, R.W., and Calos, M.P. (1984). Deletion of plasmid sequences during Saccharomyces cerevisiae transformation. J. Bact. 159, 1065-1067.

Lebkowski, J.S., DuBridge, R.B., Antell, E.A., Greisen, K.S., and Calos, M.P. (1984). Transfected DNA is mutated in monkey, mouse, and human cells. Mol. Cell. Biol. 4, 1951-1960.

Miller, J.H., Lebkowski, J.S., Greisen, K.S., and Calos, M.P. (1984). Specificity of mutations induced in transfected DNA by mammalian cells. EMBO J. 13, 3117-3121.

Lebkowski, J.S., Botchan, M.R., and Calos, M.P. (1983). Spontaneous mutation of DNA transfected into mammalian cells. In Cellular Responses to DNA Damage, UCLA Symposia on Molecular and Cellular Biology, New Series, Vol. 11, eds. Friedberg, E.C. and Bridges, B., Alan R. Liss, New York, pp. 603-614.

Lebkowski, J.S., Clancy, S., and Calos, M.P. (1985). SV40 replication in adenovirus-transformed human cells antagonizes gene expression. Nature 317, 169-171.

DuBridge, R.B., Lusky, M., Botchan, M.R., and Calos, M.P. (1985). Amplification of a bovine papilloma virus-simian virus 40 chimera. J. Virol. 56, 625-627.

Lebkowski, J.S., Clancy, S., Miller, J.H., and Calos, M.P. (1985). The lacI shuttle: Rapid analysis of the mutagenic specificity of ultraviolet light in human cells. Proc. Natl. Acad. Sci. 82, 8606-8610.

Lebkowski, J.S., Miller, J.H., and Calos, M.P. (1986). Determination of DNA sequence changes induced by ethyl methanesulfonate in human cells, using a shuttle vector system. Mol. Cell. Biol. 6, 1838-1842.

Calos, M.P. (1986). Mutation of autonomously replicating plasmids. In Gene Transfer, ed. R. Kucherlapati, Plenum Press, New York, pp. 243-261.

DuBridge, R.B., Tang, P., Hsia, H.C., Leong, P.-M., Miller, J.H., and Calos, M.P. (1987). Analysis of mutation in human cells using an Epstein-Barr virus shuttle system. Mol. Cell. Biol. 7, 379-387.

DuBridge, R.B. and Calos, M.P. (1987). Molecular approaches to the study of mutation in human cells. Trends in Genetics, 3, 293-297.

Van Den Berg, D.J., DuBridge, R.B., and Calos, M.P. (1987). EBV-lacI shuttle vectors: Addition of a selection for LacI mutants. In Banbury Report 28: Mammalian Cell Mutagenesis, pp. 307-314.

Miller, J.H., and Calos, M.P. (1987). Gene Transfer Vectors for Mammalian Cells. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York. (Book)

DuBridge, R.B. and Calos, M.P. (1988). Recombinant shuttle vectors for the study of mutation in human cells. Mutagenesis 3, 1-9.

Heinzel, S.S., Krysan, P.J., Calos, M.P., and DuBridge, R.B. (1988). Use of simian virus 40 replication to amplify Epstein-Barr virus shuttle vectors in human cells. J. Virol. 62, 3738-3746.

Calos, M.P. (1988). Use of lacI shuttle systems for analysis of mutation in human cells. In DNA Repair, Vol. 3, eds. Friedberg, E.C. and Hanawalt, P.C., Marcel Dekker, New York, chapter 13, pp. 277-294.

Haase, S.B., Heinzel, S.S., Krysan, P.J., and Calos, M.P. (1989). Improved EBV shuttle vectors. Mutation Research 220. 125-132.

Hsia, H.C., Lebkowski, J.S., Leong, P.M., Calos, M.P., and Miller, J.H. (1989). Comparison of ultraviolet light mutagenesis of the lacI gene in E. coli and in human 293 cells. J. Mol. Biol. 205, 103-113.

Krysan, P.J., Haase, S.B., and Calos, M.P. (1989). Isolation of human sequences which replicate autonomously in human cells. Mol. Cell. Biol., 9, 1026-1033.

Calos, M.P. (1990). The molecular biology of mutation in human cells: From shuttle vectors to artificial chromosomes. (Eleventh Bristol-Myers Symp. On Cancer Research), In The Cellular and Molecular Biology of Human Carcinogenesis, Chapter 7, pp.139-151.

Krysan, P.J. and Calos, M.P. (1991). Replication initiates at multiple locations on an autonomously replicating plasmid in human cells. Mol. Cell. Biol. 11, 1464-1472.

Heinzel, S.S., Krysan, P.J., Tran, C.T., and Calos, M.P. (1991). Autonomous replication in human cells is affected by the size and the source of the DNA. Mol. Cell. Biol. 11, 2263-2272.

Haase, S.B. and Calos, M.P. (1991). Replication control of autonomously replicating human sequences. Nucleic Acids Res. 19, 5053-5058.

Caddle, M.S. and Calos, M.P. (1992). Analysis of the autonomous replication behavior in human cells of the dihydrofolate reductase putative chromosomal origin of replication. Nucleic Acids Res. 20,5971-5978.

Tran, C.T., Caddle, M.S., and Calos, M.P. (1993). The replication behavior of Saccharomyces cerevisiae DNA in human cells. Chromosoma 102, 129-136.

Krysan, P.J., Smith, J.G., and Calos, M.P. (1993). Autonomous replication in human cells of multimers of specific human and bacterial DNA sequences. Mol. Cell. Biol., 13, 2688 - 2696.

Krysan, P.J. and Calos, M.P. (1993). Epstein-Barr virus-based vectors that replicate in rodent cells. Gene 136, 137-144.

Caddle, M.S. and Calos, M.P. (1994). Specific initiation at an origin of replication from Schizosaccharomyces pombe. Mol. Cell. Biol. 14, 1796-1805.

Haase, S.B., Heinzel, S.S., and Calos, M.P. (1994). Transcription inhibits the replication of autonomously replicating plasmids in human cells. Mol. Cell. Biol. 14 , 2516-2524.

Wohlgemuth, J.G., Bulboaca, G.H., Moghadam, M., Caddle, M.S., and Calos, M.P. (1994). Physical mapping of origins of replication in Schizosaccaromyces pombe. Mol. Biol. Cell 5, 839-849.

Smith, J.G. and Calos, M.P. (1995). Autonomous replication in Drosophila melanogaster tissue culture cells. Chromosoma 103, 597-605.

Smith, J.G., Caddle, M.S., Bulboaca, G.H., Wohlgemuth, J.G., Baum, M., Clarke, L., and Calos, M.P. (1995). Replication of centromere II of Schizosaccaromyces pombe. Mol. Cell. Biol. 14, 5165-5172.

Wohlgemuth, J.G., Kang, S.., Bulboaca, G.., Nawotka, K.., and Calos, M.P. (1996). Long-term gene expression from autonomously replicating vectors in mammalian cells. Gene Therapy 3, 503-512.

Calos, M.P. (1996). The potential of extrachromosomal replicating vectors in gene therapy. Trends in Genetics 12, 463-466.

Sclimenti, C.R. and Calos, M.P. (1998). Assaying extrachromosomal gene therapy vectors that carry persistence/replication elements. Advanced Drug Delivery Rev. 30, 13-21.

Bulboaca, G.H., Deng, H., Dewhurst, S., and Calos, M.P. (1998). Telomeric sequences from human herpesvirus 6 do not mediate nuclear retention of episomal DNA in human cells. Archives of Virol. 143, 563-570.

Calos, M.P. (1998). Stability without a centromere. Proc. Natl. Acad. Sci. 95, 4084-4085.

Sclimenti, C.R. and Calos, M.P. (1998) Epstein-Barr virus vectors for gene expression and transfer. Current Opinion Biotechnology 9, 476-479.

Phillips, J.E., Thyagarajan, B., and Calos, M.P. (1999). Epstein-Barr virus plasmid model system for analyzing recombination in human cells. Plasmid 41, 198-206.

Phillips, J.E. and Calos, M.P. (1999). Effects of homology length and donor vector arrangement on the efficiency of double-strand break-mediated recombination in human cells. Somat. Cell and Molec. Genet. 25, 91-100.

Phillips, J.E and Calos, M.P (2000). Transfection of DNA into mammalian cells in culture. In: Encyclopedia of Life Sciences. London: Nature Publishing Group. http://www.els.net/

Thyagarajan, B., Guimaraes, J., Groth, A.C. and Calos, M.P. (2000). Mammalian genomes contain active recombinase recognition sites. Gene 244, 47-54.

Groth, A.C., Olivares, E.C., Thyagarajan, B., and Calos, M.P. (2000). A phage integrase directs efficient site-specific integration in human cells. Proc. Natl. Acad. Sci. 97, 5995-6000.

Sclimenti, C.R., Baba, E.J., and Calos, M.P. (2000). An extrachromosomal tetracyline-regulatable system for mammalian cells. Nucleic Acids Res. 28, e80.

Thyagarajan, B., Phillips, J.E., and Calos, M.P. (2001). Gene delivery by viruses. In: Encyclopedia of Life Sciences. London: Nature Publishing Group. http://www.els.net/

Thyagarajan, B., Olivares, E.C., Hollis, R.P., Ginsburg, D.S., & Calos, M.P. (2001). Site-specific genomic integration in mammalian. cells mediated by phage φC31 integrase. Mol. Cell. Biol. 21, 3926-3934.

Stoll, S., Sclimenti, C.R., Baba, E.J., Meuse, L., Kay, M.A., & Calos, M.P. (2001). Epstein-Barr Virus/ human vector provides high-level long-term expression of α1-antitrypsin in mice. Molecular Therapy 4, 122-129.

Olivares, E.C., Hollis, R.P., and Calos, M.P. (2001). Phage R4 integrase mediates site-specific integration in human cells. Gene 278, 167-176.

Sclimenti, C.R., Thyagarajan, B., and Calos, M.P. (2001). Directed evolution of a recombinase for improved genomic integration at a native human sequence. Nucleic Acids Res. 29, 5044-5051.

Stoll, S.M., Ginsburg, D.S., and Calos M.P. (2002). Phage TP901-1 site-specific integrase functions in human cells. J. Bacteriol. 184, 3657-3663.

Stoll, S.M. and Calos, M.P. (2002). Extrachromosomal plasmid vectors for gene therapy. Curr. Opinion Mol. Therapeutics 4, 299-305.

Ortiz-Urda, S., Thyagarajan, B., Keene, D.R., Lin, Q., Fang, M., Calos, M.P., & Khavari, P. A. (2002). Stable nonviral genetic correction of inherited human skin disease. Nature Med. 8, 1166-1170.

Olivares, E.C., Hollis, R.P., Chalberg, T.W., Meuse, L., Kay, M.A., and Calos, M.P. (2002). Site-specific genomic integration produces therapeutic factor IX levels in mice. Nature Biotech. 20, 1124-1128.

Sclimenti, C.R., Neviaser, A.S., Baba, E.J., Meuse, L., Kay, M.A., and Calos, M.P. (2003). Epstein-Barr virus vectors provide prolonged robust factor IX expression in mice. Biotechnol. Prog. 19, 144-151.

Ortiz-Urda, S., Thyagarajan, B., Keene, D., Lin, Q., Calos, M.P., & Khavari, P. A. (2003). φC31 integrase-mediated nonviral genetic correction of junctional epidermolysis bullosa. Human Gene Therapy, 14, 923-928.

Portlock, J.L. and Calos, M.P. (2003). Site-specific genomic strategies for gene therapy. Curr. Opin. Molec. Therapeutics 5, 376- 382.

Hollis, R.P., Stoll, S.M., Lin, J., Chen-Tsai, Y., and Calos, M.P. (2003). Phage integrases for construction and manipulation of transgenic mammals. Reprod. Biol. and Endocrinol., 1:79.

Stoll, S.M., Meuse, L., Kay, M.A., and Calos, M.P. (2003). Role of EBV and genomic sequences in gene expression from extrachromosomal gene therapy vectors in mouse liver. Gene Therapy and Molecular Biology 7, 211-219.

Olivares, E.C. and Calos, M.P. (2003). Phage φC31 integrase-mediated site-specific integration for gene therapy. Gene Therapy and Regulation 2, 103-120.

Groth, A. C. and Calos, M.P. (2004). Phage integrases: biology and applications. J. Mol. Biol. 335, 667-678.

Groth, A.C., Fish, M., Nusse, R., and Calos, M.P. (2004). Construction of transgenic Drosophila by using the site-specific integrase from phage φC31. Genetics 166, 1775-1782.

Dorigo, O., Gil, J.S., Gallaher, S.D., Tan, B.T., Castro, M.G., Lowenstein, P.R., Calos, M.P., and Berk, A.J. (2004). Development of a novel helper dependent adenovirus-Epstein-Barr virus hybrid system for the stable transformation of mammalian cells. J. Virol. 78, 6556-6566.

Quenneville, S.P., Chapdelaine, P., Rousseau, J., Beaulieu, J., Caron, N., Skuk, D., Mills, P., Olivares, E.C., Calos, M.P., and Tremblay, J.P. (2004). Nucleofection of muscle-derived stem cells and myoblasts with fC31 integrase: stable expression of a full-length-dystrophin fusion gene by human myoblasts. Molec. Ther. 10, 679-687

Held, P.K, Olivares, E.C., Aguilar, C.P., Finegold, M., Calos, M.P., and Grompe M. (2005). In vivo correction of murine hereditary tyrosinemia type I by φC31 integrase-mediated gene delivery. Molec. Ther. 11, 399-408.

Chalberg, T.W., Genise, H., Vollrath, D., and Calos, M.P. (2005). φC31 integrase confers genomic integration and long-term transgene expression in rat retina. Investigative Ophthalmology & Visual Science 46, 2140-2146.

Chalberg, T., Phillips, J.E and Calos, M.P. (2005). Transfection of DNA into mammalian cells in culture. In: Encyclopedia of Life Sciences. London: Nature Publishing Group. http://www.els.net/

Ginsburg, D., Thyagarajan, B., Phillips, J.E., and Calos, M.P. (2005). Gene delivery by viruses. In: Encyclopedia of Life Sciences. London: Nature Publishing Group. http://www.els.net/

Thyagarajan, B. and Calos, M.P. (2005). Site-specific integration for high-level protein production in mammalian cells. In Methods in Molecular Biology, vol. 308: Therapeutic Proteins: Methods and Protocols, Chapter 9, pp. 99-106 (C M Smales and D C James, eds.), Humana Press, Totowa, NJ.

Ginsburg, D. and Calos, M.P. (2005). Site-specific integration for prolonged expression. In Non-viral Vectors for Gene Therapy, Second Edition, Part II (L. Huang, M. Hung, and E. Wagner, eds.) Adv. Genetics. Vol. 54. 177-185.

Bertoni, C., Jarrahian, S., Wheeler, T.M., Li, Y., Olivares, E.C., Calos, M.P., and Rando, T.A. (2006). Enhancement of plasmid-mediated gene therapy for Duchenne muscular dystrophy by directed plasmid integration. Proc. Natl. Acad. Sci. 103, 419-424.

Chalberg, T.W., Portlock, J.L., Olivares, E.C., Thyagarajan, B., Kirby, P., Hoelters, J., Hillman, R.T., and Calos, M.P. (2006). Integration specificity of phage φC31 integrase in the human genome. J. Mol. Biol. 357, 28-48.

Ishikawa,Y., Tanaka, N., Murakami, K., Kugoh, H., Oshimura, M., Calos, M.P., and Sugamura, K. (2006). Genomic integration of the common cytokine receptor gamma chain in human T cell lines mediated by phage φC31 integrase. J. Gene Med. 8, 646 - 653.

Keravala, A., Groth, A.C., Jarrahian, S., Hoyt, J.J., Thyagarajan, B.T., Kirby, P., and Calos, M.P. (2006). A diversity of serine phage integrases mediate site-specific recombination in mammalian cells. Mol. Genet. Genomics 276, 135 - 146.

Keravala, A., Portlock, J.L., Nash, J., Robbins, P., and Calos, M.P. (2006). φC31 integrase facilitates stable integration in synovial cells in vitro and enhances gene expression in vivo. J. Gene Med. 8, 1008 – 1017.

Chalberg, T., Vankov, A., Molnar, F., Butterwick, A.F., Huie, P., Calos, M.P., and Palanker, D. (2006). Gene transfer to rabbit retina with electron-avalanche transfection. Investigative Ophthalmology & Visual Science 47, 4083 - 4090.

Portlock, J.L., Keravala, A., Bertoni, C., Lee, S., Rando, T., and Calos, M.P. (2006). Long-term increase in mVEGF164 in mouse hindlimb muscle mediated by phage φC31 integrase after non-viral DNA delivery. Human Gene Therapy 17, 871 - 876.

Calos, M.P. (2006). The φC31 integrase system for gene therapy. Current Gene Therapy 6, 633 - 645.

Fish, M.P., Groth, A.C., Calos, M.P., and Nusse, R. (2007). Creating transgenic Drosophila by microinjecting the site-specific φC31 integrase mRNA and a transgene-containing donor plasmid. Nature Protocols, doi:10.1038/nprot.2007.328.

Hillman, R.T. and Calos, M.P. (2007). Site-specific integration with phage φC31 integrase. In Gene Transfer: Delivery and Expression of DNA and RNA, A Laboratory Manual. (T. Friedmann and J. Rossi, eds.) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. Chapter 65, 653 - 660.

Keravala, A. and Calos, M.P. (2007). Site-specific chromosomal integration mediated by φC31 integrase. Methods in Molecular Biology 435: Chromosomal Mutagenesis. (G. D. Davis and K. J. Kayser, eds.) Humana Press, Totowa, NJ, Chapter 12, 165-173.

Keravala, A., Ormond, B.K., Palmer, T.D., and Calos, M.P. (2008). Long-term transgene expression in mouse neural progenitor cells modified with φC31 integrase. J. Neuroscience Meth. 173, 299-305.

Gabrovsky, V. and Calos, M.P. (2008). Factoring nonviral gene therapy into a cure for hemophilia A. Current Opinion in Molecular Therapeutics 10, 464-470.

Woodard, L.E. and Calos, M.P. (2008). Nonviral genome modification strategies for gene therapy: Transposon, integrase, and homologous recombination systems. In Gene and Cell Therapy: Therapeutic Mechanisms and Strategies, 3rd Edition. (N.S. Templeton, ed.) Taylor & Francis Group, CRC Press, Boca Raton, FL. Chapter 31, 677-691.

Keravala, A., Lee, S., Thyagarajan, B., Olivares, E.C., Gabrovsky, V., Woodard, L.E. and Calos, M.P. (2009). Mutants of phiC31 integrase with enhanced efficiency and specificity. Molecular Therapy 17, 112-120.

Jung, W.E. and Calos, M.P. (2009). PhiC31 integrase for modification of stem cells. In Emerging Technology Platforms for Stem Cells, Chapter 20, (U. Lakshmipathy, J.D. Chesnut, and B. Thyagaragan, eds.) John Wiley and Sons, Inc.

Woodard, L.E., Hillman, R.T., Keravala, A., Lee, S., and Calos, M.P. (2010) Effect of nuclear localization and hydrodynamic delivery-induced cell division on φC31 integrase activity. Gene Therapy 17, 217-226.

Woodard, L.E. and Calos, M.P. (2010). DNA integrating vectors (transposon, integrase). In A Guide to Human Gene Therapy, Chapter 8, (R.W. Herzog and S. Zolotukhin, eds.) World Scientific Publishing Co., pp. 123 - 138.

Woodard, L.E., Keravala, A., Jung, W.E., Wapinski, O., Yang, Q., Felsher, D.W., and Calos, M.P. (2010). Impact of hydrodynamic injection and phiC31 integrase on tumor latency in a mouse model of MYC-induced hepatocellular carcinoma. PLoS One, 5, e11367.

Chavez, C.L., Keravala, A., Woodard, L.E., Hillman, R.T., Stowe, T.R., Chu, J.N., and Calos, M.P. (2010). Kinetics and longevity of ?C31 integrase in mouse liver and cultured cells. Human Gene Therapy 21, 1287 – 1297.

Ito, M., Yamanouchi, K., Naito, K., Calos, M.P. and Tojo, H. (2011). Site-specific integration of transgene targeting an endogenous lox-like site in early mouse embryos. J. Appl. Genet. 52, 89 -94.

Keravala, A., Chavez, C.L., Hu, G., Woodard, L.E., Monahan, P.E., and Calos, M.P. (2011) Long-term phenotypic correction in factor IX knockout mice by using phiC31 integrase-mediated gene therapy. Gene Therapy 18, 842-848.

Karow, M. and Calos, M.P. (2011). The therapeutic potential of phiC31 integrase as a gene therapy system. Expert Opin. Biol. Ther. 11, 1287-1296.

Chavez, C.L. and Calos, M.P. (2011). Therapeutic applications of the phiC31 integrase system. Current Gene Therapy 11, 375 - 381.

Karow, M., Chavez, C.L., Farruggio, A.P., Geisinger, J.M., Keravala, A., Jung, W.E., Lan, F., Wu, J.C., Chen-Tsai, Y., and Calos, M.P. (2011) Site-specific recombinase strategy to create iPS cells efficiently with plasmid DNA. Stem Cells 29, 1696 - 1704.

Chavez, C.L., Keravala, A., Chu, J.N., Farruggio, A.P., Gabrovsky, V.E., Voorberg, J., and Calos, M.P. (2012). Long-term expression of human coagulation factor VIII in a tolerant mouse model using the phiC31 integrase system. Human Gene Therapy 23, 390 – 398.

Hillman RT, Calos MP. (2012) Site-specific integration with bacteriophage ΦC31 integrase. Cold Spring Harb Protoc; May:2012 (5).

Geisinger, J.M. and Calos, M.P. (2012). Site-specific recombination using phiC31 integrase. In “Site-directed insertion of transgenes.”, (P. Duchateau and S. Renault, eds.) Topics in Current Genetics 23, Springer, Chapter 8, pp. 211-239.

Lan, F., Liu, J., Narsinh, K., Hu, S., Han, L., Lee, A.S., Karow, M., Nguyen, P.K., Nag, D., Calos, M.P., Robbins, R.C., and Wu, J.C. (2012). Safe Genetic Modification of Cardiac Stem Cells Using a Site-Specific Integration Technique. Circulation 126 (suppl 1): S20 – S28.

Farruggio, A.P., Chavez, C.L., Mikell, C.L., and Calos, M.P. (2012). Efficient reversal of phiC31 integrase recombination in mammalian cells. Biotechnol. J. 7, 1332 - 1336.



 
 
 
Maintained by Tawny Neal
Last updated on
November 12, 2012