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Receptor-Ligand Analysis

1. Single binding site: Assume only one ligand binds to each receptor

Usually in an experiment [L] is varied and measures [B]. The desire is to estimate K_D (or its inverse K_A = 1/K_D) and [R_T]. If you know the total receptor protein molar concentration [P], then [R_T]/[P] = number of binding sites/molecule of protein.

C.4a

Receptor-Ligand Analysis

1. Single binding site: Assume only one ligand binds to each receptor

Usually in an experiment [L] is varied and measures [B]. The desire is to estimate KD (or its inverse KA = 1/KD) and [RT]. If you know the total receptor protein molar concentration [P], then [RT]/[P] = number of binding sites/molecule of protein.

Usually in an experiment [L] is varied and measures [B]. The desire is to estimate K_D (or its inverse K_A = 1/K_D) and [R_T]. If you know the total receptor protein molar concentration [P], then [R_T]/[P] = number of binding sites/molecule of protein.