Construction of a retroviral cDNA version of the int-1 mammary oncogene and its expression in vitro.
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Title | Construction of a retroviral cDNA version of the int-1 mammary oncogene and its expression in vitro. |
Publication Type | Journal Article |
Year of Publication | 1986 |
Authors | Rijsewijk FA, van Lohuizen M, van Ooyen A, Nusse R |
Journal | Nucleic Acids Res |
Volume | 14 |
Pagination | 693–702 |
Date Published | Jan |
ISSN | 0305-1048 (Print); 0305-1048 (Linking) |
Abstract | The int-1 mammary oncogene is activated by proviruses of the Mouse Mammary Tumor Virus in many different mammary tumors. We have inserted a genomic fragment containing the protein-encoding domain of the gene into the retroviral shuttle vector pZIPneoSV(X)1. After one round of virus replication we recovered recombinant proviral DNA containing a correctly spliced copy of int-1. In vitro transcription of this cDNA version of int-1 using SP6 polymerase and translation in a reticulocyte lysate yielded a protein of approximately 37,000 daltons. High expression of int-1 in NIH-3T3 cells infected with recombinant virus did not lead to morphological transformation. |