The nucleotide sequence of the human int-1 mammary oncogene; evolutionary conservation of coding and non-coding sequences.
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Title | The nucleotide sequence of the human int-1 mammary oncogene; evolutionary conservation of coding and non-coding sequences. |
Publication Type | Journal Article |
Year of Publication | 1985 |
Authors | van Ooyen A, Kwee V, Nusse R |
Journal | EMBO J |
Volume | 4 |
Pagination | 2905–2909 |
Date Published | Nov |
ISSN | 0261-4189 (Print); 0261-4189 (Linking) |
Abstract | The mouse mammary tumor virus can induce mammary tumors in mice by proviral activation of an evolutionarily conserved cellular oncogene called int-1. Here we present the nucleotide sequence of the human homologue of int-1, and compare it with the mouse gene. Like the mouse gene, the human homologue contains a reading frame of 370 amino acids, with only four substitutions. The amino acid changes are all in the hydrophobic leader domain of the int-1 encoded protein, and do not significantly alter its hydropathic index. The conservation between the mouse and the human int-1 genes is not restricted to exons; extensive parts of the introns are also homologous. Thus, int-1 ranks among the most conserved genes known, a property shared with other oncogenes. |