Open Positions

Postdoc position 1: Understanding Transcriptional Heterogeneity in Human Heart

We are currently seeking a postdoctoral fellow to lead the experimental work on understanding transcriptional heterogeneity in human heart with state-of-art sequencing technologies. Our project aims to map all full-length transcript isoforms in single heart cells. You will lead the development of novel single-cell transcriptomics methods based on long-read sequencing. This project will be strongly supported by a dedicated computational biologist from our lab.

Candidates should have a Ph.D. in a relevant field with strong expertise in RNA biology and sequencing technologies. Experience in cardiac biology and in cultivating or isolating heart cells will be a bonus but is not a requirement.

Postdoc position 2: Determining Immunological Basis of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome

We are currently seeking a postdoctoral fellow to lead a project on determining the immunological basis of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS). The project aims to understand ME/CFS etiology by applying state-of-the-art single-cell transcriptomics to blood samples from ME/CFS patients. You will lead the study design and perform experiments, including large-scale single-cell transcriptomics in human T-cells and other cells of the immune system. The data analysis and interpretation will be supported by a bioinformatician from the lab.

Candidates should have a Ph.D. in a relevant field with strong expertise in immunology and single-cell RNA-seq. Knowledge and skills in analyzing NGS data will be a bonus but not required.

Postdoc position 3: Developing Precision CRISPR Editing Technologies in Human Cells

We are looking for a highly motivated postdoctoral fellow to work on developing precision CRISPR editing technologies in human cell lines in our systems genetics lab at the Stanford Genome Technology Center. We have recently described a system which improves the efficiency of homology directed repair (HDR) by an order of magnitude by actively recruiting donor DNA to CRISPR-mediated double-strand breaks (Roy, Smith, Vonesch et al. Nat Biotech. 2018). This allows for highly multiplexed, systematic genome editing in the model eukaryote S. cerevisiae to quantitatively measure the effects of all genetic variants in this species. We seek a candidate interested in developing similar HDR-boosting technologies for human cells, including donor recruitment as well as additional approaches, to facilitate CRISPR editing screens in several types of human cell lines. This will enable identification of causal variants and may also have applications for gene and cell therapies. The candidate would be working closely with the team who established the donor recruitment and multiplexed editing technologies in yeast to guide the development of HDR-enhancing systems in human cells.

Candidates should have a Ph.D. in a relevant field with required experience in human tissue culture, transfection, cell line construction, and constructing vectors for mammalian cells. Experience in genome editing (CRISPR, Talens, Zinc Fingers, etc), next generation sequencing analysis and background in DNA repair will be beneficial but not required.

General Requirements

The appointed candidate should be highly motivated, creative, and well organized. Excellent communication skills, and fluency in written and verbal English, are essential. S/he should be capable of working independently but also enjoy being part of an interdisciplinary, collaborative, and international team.

Appointment term and benefits

All postdoctoral fellows have an initial appointment term of two years and this may be extended to five years in total. Benefits for postdoctoral fellows can be found here.

Contacts

Please submit your application documents directly to Prof. Lars Steinmetz (larsms [at] stanford.edu), including your CV and a brief motivation letter and contacts for two references.