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Laboratory protocols

• A. Zulli, A. Paulos, B. Hughes, D. Duong, A. Bidwell, P. Thana, M. Wolfe, A. B. Boehm. High throughput wild type measles quantification in settled solids using digital RT-PCR. protocols.io. Link.
• Laura Roldan, Alexandria B Boehm, Camila Van Oost. 2024. Protocol to study the adsorption of viruses spiked into primary sludge samples: RNA Extraction and Inhibitor Removal. protocols.io. Link.
• Laura Roldan, Camila Van Oost, Alexandria B Boehm. 2024. Protocol to study the adsorption of viruses spiked into primary sludge samples: Purification of lab-grown viruses and preanalytical process. protocols.io. Link.
• Meghan M. Shea, Alexandria B Boehm 2023. DNA Extraction Protocol from Sterivex Filters. protocols.io Link.
• Meghan M. Shea, Alexandria B Boehm 2023. Environmental DNA (eDNA) COI PCR Amplification and Gel Electrophoresis Protocol. protocols.io Link.
• Meghan M. Shea, Alexandria B Boehm 2023. Coastal Environmental DNA Sampling & Gravity Filtration Protocol. protocols.io Link.
• Vikram Chan-Herur, David Catoe, Dorothea Duong, Yi-Chieh Wu, Alexandria B Boehm, Bradley J White, Marlene K Wolfe 2023. SARS-CoV-2 Whole Genome Amplicon Sequencing from Wastewater Solids or Liquid Wastewater. protocols.io Link.
• B. Hughes, B. J. White, M. K. Wolfe, A. B. Boehm. 2022. Quantification of SARS-CoV-2 variant mutations (HV69-70, E484K/N501Y, del156-157/R158G, del143-145, LPPA24S, S:477-505, and ORF1a Del 141-143) in settled solids using digital RT-PCR. protocols.io Link.
• Aaron Topol, Marlene Wolfe, Brad White, Krista Wigginton, Alexandria B Boehm 2021. High Throughput pre-analytical processing of wastewater settled solids for SARS-CoV-2 RNA analyses. protocols.io Link.
• Aaron Topol, Marlene Wolfe, Krista Wigginton, Bradley White, Alexandria B Boehm 2021. High Throughput RNA Extraction and PCR Inhibitor Removal of Settled Solids for Wastewater Surveillance of SARS-CoV-2 RNA. protocols.io Link.
• Aaron Topol, Marlene Wolfe, Brad White, Krista Wigginton, Alexandria B. Boehm. 2021. High Throughput SARS-COV-2, PMMOV, and BCoV quantification in settled solids using digital RT-PCR. protocols.io Link.
• K. Graham, M. Wolfe, S. Loeb, A. B. Boehm, K. R. Wigginton 2020. Concentration of viruses from wastewater influent using organic flocculation (PEG). protocols.io Link.
• S. Loeb, K. Graham, D. Catoe, M. Wolfe, A. B. Boehm, K. R. Wigginton 2020. One-Step RT-ddPCR for Detection of SARS-CoV-2, Bovine Coronavirus, and PMMoV RNA in RNA Derived from Wastewater or Primary Settled Solids . protocols.io Link.
• S. Loeb, K. Graham, M. Wolfe, K. R. Wigginton, A. B. Boehm 2020. Extraction of RNA from Wastewater Primary Solids Using a Direct Extraction Method for Downstream SARS-CoV-2 RNA Quantification. protocols.io Link.
• Collin Closek, Anni Djurhuus, Katie Pitz, Ryan Kelly, Reiko Michisaki, Kristine Walz, Hilary Starks, Francisco Chavez, Alexandria Boehm, Mya Breitbart 2018. Environmental DNA (eDNA) COI metabarcoding Illumina MiSeq NGS PCR Protocol. protocols.io Link.
• Collin Closek, Anni Djurhuus, Katie Pitz, Ryan Kelly, Reiko Michisaki, Kristine Walz, Hilary Starks, Francisco Chavez, Alexandria Boehm, Mya Breitbart 2018. Environmental DNA (eDNA) 12S metabarcoding Illumina MiSeq NGS PCR Protocol. protocols.io Link.
• Collin Closek, Anni Djurhuus, Katie Pitz, Ryan Kelly, Reiko Michisaki, Kristine Walz, Hilary Starks, Francisco Chavez, Alexandria Boehm, Mya Breitbart 2018. Environmental DNA (eDNA) 18S metabarcoding Illumina MiSeq NGS PCR Protocol. protocols.io Link.
• Collin Closek, Anni Djurhuus, Katie Pitz, Ryan Kelly, Reiko Michisaki, Kristine Walz, Hilary Starks, Francisco Chavez, Alexandria Boehm, Mya Breitbart 2018. Environmental DNA (eDNA) 16S metabarcoding Illumina MiSeq NGS PCR Protocol. protocols.io Link.