LOCUS       pBAD-pelB-   4993 bp    DNA   circular            22-JUN-2004
DEFINITION  8 mutation of hRLuc with pelB and no myc epitope on vector, A55TC124AS130AK136RA143MM185VM253LS287L.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|336673945|
COMMENT     VNTDBDATE|341998728|
COMMENT     VNTNAME|pBAD-pelB-Rluc8|
COMMENT     VNTAUTHORNAME|Loening|
COMMENT     VNTAUTHOREML|loening@ucla.edu|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
FEATURES             Location/Qualifiers
     misc_feature    67..996
                     /vntifkey="21"
                     /label=RLuc8
                     /note="hRLuc with A55T, C124A, S130A, K136R, A143M,  M185V, M253L, S287L"
     promoter        4679..4951
                     /vntifkey="30"
                     /label=pBAD
                     /note="araBAD promoter region"
     misc_feature    1003..1020
                     /vntifkey="21"
                     /label=Polyhistidine\tag
     misc_feature    1126..1283
                     /vntifkey="21"
                     /label=transcript\term
                     /note="rmB transcription termination region"
     mRNA            1562..2422
                     /vntifkey="54"
                     /label=Amp
                     /note="Ampicillin ORF"
     rep_origin      2567..3240
                     /vntifkey="33"
                     /label=ORI
                     /note="pBR322 origin"
     mRNA            complement(3771..4649)
                     /vntifkey="54"
                     /label=AraC
                     /note="AraC ORF"
     sig_peptide     1..66
                     /vntifkey="94"
                     /label=pelB
                     /note="pelB signal peptide"
BASE COUNT     1241 a      1307 c      1268 g      1177 t 
ORIGIN
        1 atgaaatacc tattgcctac ggcagccgct ggattgttat tactcgcggc ccaaccggcc 
       61 atggccgctt ccaaggtgta cgaccccgag caacgcaaac gcatgatcac tgggcctcag 
      121 tggtgggctc gctgcaagca aatgaacgtg ctggactcct tcatcaacta ctatgattcc 
      181 gagaagcacg ccgagaacgc cgtgattttt ctgcatggta acgctacctc cagctacctg 
      241 tggaggcacg tcgtgcctca catcgagccc gtggctagat gcatcatccc tgatctgatc 
      301 ggaatgggta agtccggcaa gagcgggaat ggctcatatc gcctcctgga tcactacaag 
      361 tacctcaccg cttggttcga gctgctgaac cttccaaaga aaatcatctt tgtgggccac 
      421 gactgggggg ctgctctggc ctttcactac gcctacgagc accaagacag gatcaaggcc 
      481 atcgtccata tggagagtgt cgtggacgtg atcgagtcct gggacgagtg gcctgacatc 
      541 gaggaggata tcgccctgat caagagcgaa gagggcgaga aaatggtgct tgagaataac 
      601 ttcttcgtcg agaccgtgct cccaagcaag atcatgcgga aactggagcc tgaggagttc 
      661 gctgcctacc tggagccatt caaggagaag ggcgaggtta gacggcctac cctctcctgg 
      721 cctcgcgaga tccctctcgt taagggaggc aagcccgacg tcgtccagat tgtccgcaac 
      781 tacaacgcct accttcgggc cagcgacgat ctgcctaagc tgttcatcga gtccgaccct 
      841 gggttctttt ccaacgctat tgtcgaggga gctaagaagt tccctaacac cgagttcgtg 
      901 aaggtgaagg gcctccactt cctccaggag gacgctccag atgaaatggg taagtacatc 
      961 aagagcttcg tggagcgcgt gctgaagaac gagcaggtcg accatcatca tcatcatcat 
     1021 tgagtttaaa cggtctccag cttggctgtt ttggcggatg agagaagatt ttcagcctga 
     1081 tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta 
     1141 gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg 
     1201 gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag 
     1261 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg 
     1321 agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg 
     1381 cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg 
     1441 gatggccttt ttgcgtttct acaaactctt ttgtttattt ttctaaatac attcaaatat 
     1501 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 
     1561 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc 
     1621 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc 
     1681 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc 
     1741 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc 
     1801 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt 
     1861 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt 
     1921 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat 
     1981 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct 
     2041 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat 
     2101 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc 
     2161 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg 
     2221 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc 
     2281 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta 
     2341 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc 
     2401 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga 
     2461 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 
     2521 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat 
     2581 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 
     2641 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 
     2701 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt 
     2761 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 
     2821 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 
     2881 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 
     2941 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 
     3001 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 
     3061 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 
     3121 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 
     3181 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat 
     3241 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc 
     3301 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga 
     3361 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg 
     3421 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta 
     3481 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc 
     3541 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc 
     3601 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagca gatcaattcg 
     3661 cgcgcgaagg cgaagcggca tgcataatgt gcctgtcaaa tggacgaagc agggattctg 
     3721 caaaccctat gctactccgt caagccgtca attgtctgat tcgttaccaa ttatgacaac 
     3781 ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct cgggctggcc 
     3841 ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga 
     3901 ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt 
     3961 tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag atgtgacaga 
     4021 cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc 
     4081 aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg tggatggagc 
     4141 gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt tatcgccagc 
     4201 agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa caggtcgctg 
     4261 aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa tattgacggc 
     4321 cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg gtgataccat 
     4381 tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa cagcaaaata 
     4441 tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg accgcgaatg 
     4501 gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa atcgagataa 
     4561 ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga gtatcccggc 
     4621 agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt cagagaagaa 
     4681 accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg gctcttctcg 
     4741 ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg gaccaaagcc 
     4801 atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca cattgattat 
     4861 ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt agcggatcct 
     4921 acctgacgct ttttatcgca actctctact gtttctccat acccgttttt tgggctaaca 
     4981 ggaggaatta acc 
//
