LOCUS       pcDNA-RLuc8.6-53        6358 bp    DNA     circular     13-JUL-2007
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|447279473|
COMMENT     VNTDBDATE|451861002|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pcDNA-RLuc8.6-535|
COMMENT     VNTAUTHORNAME|Andy Loening|
COMMENT     VNTAUTHOREML|loening@stanford.edu|
FEATURES             Location/Qualifiers
     misc_feature    1..933
                     /vntifkey="21"
                     /label=RLuc8.6-535
                     /note="hRLuc with A55T, A123S, C124A, S130A, K136R, A143M, D154M, E155G, D162E, I163L,  V185L, M253L, S287L"
     promoter        5686..6273
                     /vntifkey="29"
                     /label=Pcmv
                     /note="Human cytomegalovirus (CMV) immediate-early promoter/enhancer - Permits efficient, high-level expression of your recombinant protein"
     promoter        6317..6336
                     /vntifkey="30"
                     /label=T7
                     /note="T7 promoter/priming site - allows for in vitro transcription in the sense orientation and sequencing through the insert"
     misc_feature    1048..1065
                     /vntifkey="21"
                     /label=rev
                     /note="pcDNA3.1/BGH reverse priming site"
     polyA_signal    1054..1278
                     /vntifkey="25"
                     /label=BGH\pA
                     /note="Bovine growth hormone (BGH) polyadenylation signal - efficient transcription termination and polyadenylation of mRNA"
     rep_origin      1324..1752
                     /vntifkey="33"
                     /label=f1\ori
                     /note="f1 origin - allows rescue of single-standed DNA"
     rep_origin      1757..2100
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="SV40 early promoter and origin - allows efficient, high-level expression of the neomycin resistance gene and episomal replication in cells expressing SV40 large T antigen"
     mRNA            2162..2956
                     /vntifkey="54"
                     /label=Neomycin
                     /note="Neomycin (G418) resistance gene (ORF) - selection of stable transfectants in mammalian cells"
     polyA_signal    3130..3260
                     /vntifkey="25"
                     /label=SV40\pA
                     /note="SV40 early polyadenylation signal - efficient transcription termination and polyadenylation of mRNA"
     rep_origin      complement(3643..4323)
                     /vntifkey="33"
                     /label=pMB1
                     /note="pMB1 origin (pUC-derived) -  high copy number replication and growth in E. coli"
     mRNA            complement(4458..5318)
                     /vntifkey="54"
                     /label=Ampicillin
                     /note="Ampicillin resistance gene (beta-lactamase) (orf) - selection of vector in e.coli"
     promoter        complement(5353..5359)
                     /vntifkey="30"
                     /label=Pbla
                     /note="bla promoter (P3)"
BASE COUNT     1470 a      1668 c      1651 g      1569 t 
ORIGIN
        1 atggcttcca aggtgtacga ccccgagcaa cgcaaacgca tgatcactgg gcctcagtgg 
       61 tgggctcgct gcaagcaaat gaacgtgctg gactccttca tcaactacta tgattccgag 
      121 aagcacgccg agaacgccgt gatttttctg catggtaacg ctacctccag ctacctgtgg 
      181 aggcacgtcg tgcctcacat cgagcccgtg gctagatgca tcatccctga tctgatcgga 
      241 atgggtaagt ccggcaagag cgggaatggc tcatatcgcc tcctggatca ctacaagtac 
      301 ctcaccgctt ggttcgagct gctgaacctt ccaaagaaaa tcatctttgt gggccacgac 
      361 tgggggagcg ctctggcctt tcactacgcc tacgagcacc aagacaggat caaggccatc 
      421 gtccatatgg agagtgtcgt ggacgtgatc gagtcctgga tggggtggcc tgacatcgag 
      481 gaggagctgg ccctgatcaa gagcgaagag ggcgagaaaa tggtgcttga gaataacttc 
      541 ttcgtcgaga ccctgttgcc aagcaagatc atgcggaaac tggagcctga ggagttcgct 
      601 gcctacctgg agccattcaa ggagaagggc gaggttagac ggcctaccct ctcctggcct 
      661 cgcgagatcc ctctcgttaa gggaggcaag cccgacgtcg tccagattgt ccgcaactac 
      721 aacgcctacc ttcgggccag cgacgatctg cctaagctgt tcatcgagtc cgaccctggg 
      781 ttcttttcca acgctattgt cgagggagct aagaagttcc ctaacaccga gttcgtgaag 
      841 gtgaagggcc tccacttcct ccaggaggac gctccagatg aaatgggtaa gtacatcaag 
      901 agcttcgtgg agcgcgtgct gaagaacgag cagtaaaagc ttggtaccga gctcggatcc 
      961 actagtccag tgtggtggaa ttctgcagat atccagcaca gtggcggccg ctcgagtcta 
     1021 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg 
     1081 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 
     1141 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 
     1201 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 
     1261 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc 
     1321 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 
     1381 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 
     1441 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 
     1501 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 
     1561 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 
     1621 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 
     1681 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 
     1741 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc 
     1801 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa 
     1861 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 
     1921 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 
     1981 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc 
     2041 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct 
     2101 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg 
     2161 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 
     2221 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 
     2281 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 
     2341 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 
     2401 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 
     2461 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 
     2521 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 
     2581 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 
     2641 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga 
     2701 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 
     2761 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 
     2821 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 
     2881 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 
     2941 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac 
     3001 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 
     3061 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 
     3121 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 
     3181 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 
     3241 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg 
     3301 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 
     3361 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 
     3421 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 
     3481 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 
     3541 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 
     3601 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 
     3661 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 
     3721 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 
     3781 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 
     3841 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 
     3901 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 
     3961 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 
     4021 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 
     4081 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 
     4141 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 
     4201 agctcttgat ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag 
     4261 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 
     4321 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 
     4381 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 
     4441 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 
     4501 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 
     4561 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 
     4621 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 
     4681 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 
     4741 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 
     4801 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 
     4861 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 
     4921 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 
     4981 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 
     5041 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 
     5101 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 
     5161 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 
     5221 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 
     5281 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 
     5341 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 
     5401 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga 
     5461 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat 
     5521 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca 
     5581 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg 
     5641 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat 
     5701 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt 
     5761 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 
     5821 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 
     5881 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 
     5941 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 
     6001 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 
     6061 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 
     6121 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 
     6181 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 
     6241 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt 
     6301 actggcttat cgaaattaat acgactcact atagggagac ccaagctggc tagccacc 
//
