Machine Name
    nmr400
    Location
    Stauffer I 18B

    Hardware

   400 MHz NMR spectrometer
   Varian Direct Drive console
   Dell Precision 380

    Software
    Red Hat Linux 4.3
    VNMRJ 2.1B
    Primary Uses of Instrument
    1) 1D 1H, 13C, 19F, 31P
    2) 1D NOE
    3) 1D with variable temperature
    4) Basic 2D experiments including COSY, TOCSY, HSQC,                 HMBC
    Probes
   5mm 4-Nucleus (13C/31P-1H/19F) (Standard Probe)
       Ideal for easy detection of 31P and 19F
       Broadband detect probe, better signal-to-noise on 13C and 31P

    5mm Inverse Pulsed Field Gradient Probe
       H/X
       Tunable to X nuclei from 31P (121 MHz) to 15N (30 MHz)
       Ideal for proton detection, all basic 2D experiments

       Including:
       NOESY/ROESY/TOCSY (1H/1H)
       HMQC/HSQC/HMBC (1H/13C or 1H/15N)
       31P/1H Heteronuclear COSY/TOCSY


 


Detection of 19F or 31P
What Probe Should You Choose?
Probes for Varian 400
Setting the VT Temperature
Why Tune the Probe?
Gradient Shimming
Calibrate 1H 90* Pulse
Calibrate 13C 90* Pulse for Indirect Detect
Types of Two Dimensional Experiments
Set-Up Macros for Two Dimensional Experiments
Parameters that Affect Length of 2D Experiment
HSQC
HMQC
HMBC
NOESY
ROESY
TOCSY
Data Processing
Data Display

 

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