General inquiry regarding WNT activation
I've seen in the literature that researchers DO or DO NOT serum starve prior to treating with Wnt. I am looking into performing some Wnt3a treatments. Should I serum starve my cells prior to treating them? I will be using recombinant Wnt3a. Does it matter if using recombinant Wnt3a vs. L/Wnt3a conditioned medium?