LECTURES

The computer slide presentation for each lecture has been made into a pdf document and posted below. Because some of the presentations, which were made in PowerPoint or Keynote, used animated transitions, some slides are altered from their original form and may be difficult to read.

Dr. Elrad - 4/3/07

Introduction to the class.

This lecture includes a brief introduction to each lab.

Dr. Kool - 4/17/07

Chemistry of the ZsYellow fluorophore.

Topics discussed in this lecture:
(a) What makes a molecule fluorescent.
(b) The mechanism for formation of the ZsYellow chromophore.

Dr. Zare - 4/20/07

Collecting and interpreting fluorescence spectra.

Topics discussed in this lecture:
(a) The photochemistry behind fluorescence.
(b) The instrumentation of a spectrophotometer and spectrofluorometer.
(c) How to interpret fluorescence spectra.

Dr. Zare - 4/24/07

An introduction to single-molecule spectroscopy.

Topics discussed in this lecture:
(a) The significance of single-molecule spectroscopy.
(b) The technical challenges that single-molecule spectroscopy entails.

Dr. Altman - 4/24/07

Diffusion of proteins.

Topics discussed in this lecture:
(a) Low Reynolds number motion.
(b) Brownian motion.

Dr. Altman - 5/15/07

Modeling fluorescence correlation spectroscopy data.

This lecture discussed the analysis of computer-simulated fluorescence correlation spectroscopy data. The simulated data mimicked the data collected during the lab: Aligning a fluorescence correlation spectrometer.

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