Determination of Vm and
Km
Linear representation of the the Michaelis-Menten Equation
Lineweaver-Burke equation
this equation is written:
where
is the change in plasma concentration at a given time interval.
Sm is the average plasma concentration between two
sampling times.
A plot of
versus
1/Sm produces a straight line with slope
Km/Vm and a y-intercept at 1/Vm.
C.3a-3
