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LECTURES |
The computer slide presentation for each lecture has been made into a pdf document and posted below. Because some of the presentations, which were made in PowerPoint or Keynote, used animated transitions, some slides are altered from their original form and may be difficult to read. |
Dr. Elrad |
Introduction to the class.This lecture includes a brief introduction to each lab. |
Dr. Elrad |
The tyrosinase protein families.This lecture discusses how to identify members of a protein family, using the tyrosinase protein family as a case study. |
Dr. Kool |
Chemistry of the ZsYellow fluorophore.Topics discussed in this lecture: (a) What makes a molecule fluorescent. (b) The mechanism for formation of the ZsYellow chromophore. |
Dr. Zare |
Collecting and interpreting fluorescence spectra.Topics discussed in this lecture: (a) The photochemistry behind fluorescence. (b) The instrumentation of a spectrophotometer and spectrofluorometer. (c) How to interpret fluorescence spectra. |
Dr. Altman |
Diffusion of proteins. Topics discussed in this lecture: (a) Low Reynolds number motion. |
Dr. Altman |
Statistics of single molecule data.This lecture discussed how to analyze fluorescence data to convince yourself that fluorescent events are the result of individual molecules, and not fluorescence from multiple fluorophores arriving coincidently at the detector. |
You can also download the class lectures from when the course was taught in the Spring of 2007 at this webpage |
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