Latest News: SpliceMap -- Faster and more accurate ... read more


SpliceMap aligns RNA-seq reads to a reference genome in order to discover splicing junctions. It is at least as sensitive and specific as state-of-the art tools. Some major features of SpliceMap are

The following table gives an idea of the improvements expected from long-reads. The results are from the same 20 million 100bp RNA-seq reads cut to different lengths and mapped to the human genome (hg18). Specificity is calculated based on the number of junctions validated by ESTs. The results are unfiltered, improvements to specificity can be achieved using the optional filters.

50bp 75bp 100bp
Total Junctions 158,060 161,295 170,213
EST Validated 136,054 137,964 142,432
Specificity 86.08% 85.54% 83.68%