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Dept of Molecular Pharmacology
Dept of Microbiology & Immunology

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2. The psi-2 Packaging Line of Mann, Mulligan and Baltimore


The use of recombinant retroviruses was pioneered by Richard Mulligan and David Baltimore with the Psi-2 lines and analogous retrovirus packaging systems, based on NIH 3T3 cells (10, 11). Such helper-defective packaging lines are capable of producing all the necessary trans proteins--gag, pol, and env-- that are required for packaging, processing, reverse transcription, and integration of recombinant genomes. Those RNA molecules that have in cis the Psi packaging signal are packaged into maturing virions.




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