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Dept of Molecular Pharmacology
Dept of Microbiology & Immunology

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3. How to make a retrovirus.


Once a packaging cell line has been made, or some other means has been devised to provide the necessary packaging proteins, one must provide the retroviral RNA with the requisite cis-acting sequences. Required is sequences from the long terminal repeats at the 5' and 3' ends of the DNA.




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