Heilshorn Biomaterials Group

Selected Publications

Krajina BA, LeSavage BL*, Roth JG*, Zhu AW, Cai PC, Spakowitz AJ, Heilshorn SC. Microrheology reveals simultaneous cell-mediated matrix stiffening and fluidization that underlie breast cancer invasion. Science Advances, 2021, 7:eabe1969  » PDF    » Article
*These authors contributed equally to this work

LeSavage BL, Heilshorn SC. Defined matrices bring IBD to 3D. Nature Materials, 2021, 20:124-125  » PDF    » Article

Cai PC, Krajina BA, Kratochvil MJ, Zou L, Zhu A, Burgener EB, Bollyky P, Milla C, Webber M, Spakowitz A, Heilshorn SC. Dynamic light scattering microrheology for soft and living materials. Soft Matter, 2021, 17:1929-1939  » PDF    » Article

Hull SM*, Lindsay CD*, Brunel LG, Shiwarski DJ, Tashman JW, Roth JG, Myung D, Feinberg AW, Heilshorn SC. 3D bioprinting using UNIversal Orthogonal Network (UNION) bioinks. Advanced Functional Materials, 2021, 31, 2007983  » PDF    » Article
*These authors contributed equally to this work

Nagy N, Kaber G, Kratochvil MJ, Kuipers HF, Ruppert SM, Yadava K, Yang J, Heilshorn SC, Long SA, Pugliese A, Bollyky PL. Weekly injection of IL-2 using an injectable hydrogel reduces autoimmune diabetes incidence in NOD mice. Diabetologia, 2021, 64:152-158  » PDF    » Article

Liu M, Iosef C, Rao S, Domingo-Gonzalez R, Fu S, Snider P, Conway SJ, Umbach GS, Heilshorn SC, Dewi RE, Dahl MJ, Null DM, Albertine KH, Alvira CM. Transforming growth factor induced protein promotes NF-Kappa-B mediated angiogenesis during postnatal lung development. Am J Respir Cell Mol Biol, 2020  » PDF    » Article

Suhar RA, Marquardt LM, Song S, Buabbas H, Doulames VM, Johansson PK, Klett KC, Dewi RE, Enejder AMK, Plant GW, George PM, Heilshorn SC. Elastin-like proteins to support peripheral nerve regeneration in guidance conduits. ACS Biomater. Sci. Eng, 2020  » PDF    » Article

Kaber G, Kratochvil MJ, Burgener EB, Peltan EL, Barlow G, Yang S, Nicolls MR, Perez V, Rosser JI, Wardle AJ, Kalinowski A, Ozawa MG, Regula DP, Nagy N, Heilshorn SC, Milla CE, Rogers AJ, Bollyky PL. Hyaluronan is abundant in COVID-19 respiratory secretions. medRxiv 2020.09.11.20191692  » PDF    » Article

Guan Y, Enejder A, Wang M, Fang Z, Cui L, Chen S, Wang J, Tan Y, Wu M, Chen X, Johansson PK, Osman I, Kunimoto K, Russo P, Heilshorn SC, Peltz G. A human multi-lineage hepatic organoid model for liver fibrosis. bioRxiv 2020.09.01.278473  » PDF    » Article

Madl CM, LeSavage BL, Khariton M, Heilshorn SC. Neural progenitor cells alter chromatin organization and neurotrophin expression in response to 3D matrix degradability. Advanced Healthcare Materials, 2020, 9, 2000754  » PDF    » Article

Chen F, Lea P, Fernandes-Cunha GM, Heilshorn SC, Myung D. Bio-orthogonally crosslinked hyaluronate-collagen hydrogel for suture-free corneal defect repair. Biomaterials, 2020, 255, 120176  » PDF    » Article

Duarte Campos DF, Lindsay CD, Roth JG, LeSavage BL, Seymour AJ, Krajina BA, Ribeiro R, Costa PF, Blaeser A, Heilshorn SC. Bioprinting cell- and spheroid-laden protein-engineered hydrogels as tissue-on-chip platforms. Frontiers in Bioengineering and Biotechnology, 2020, 8:374  » PDF    » Article

Marquardt LM, Doulames VM, Wang AT, Dubbin K, Suhar RA, Kratochvil MJ, Medress ZA, Plant GW, Heilshorn SC. Designer, injectable gels to prevent transplanted Schwann cell loss during spinal cord injury therapy. Science Advances, 2020, 6(14):eaaz1039  » PDF    » Article

Ferro MP, Heilshorn SC, Owens RM. Materials for blood brain barrier modeling in vitro. Materials Science & Engineering R, 2020, 140:100522  » PDF    » Article

Madl CM, Heilshorn SC. Rapid Diels−Alder cross-linking of cell encapsulating hydrogels. Chemistry of Materials, 2019, 31:8035-8043  » PDF    » Article

Kratochvil MJ, Seymour AJ, Li TL, Pasca SP, Kuo CJ, Heilshorn SC. Engineered materials for organoid systems. Nature Reviews Materials, 2019, 4:606-622  » PDF    » Article

Yang H, Qin X, Wang H, Zhao X, Liu Y, Wo H, Liu C, Nishiga M, Chen H, Ge J, Sayed N, Abilez OJ, Ding D, Heilshorn SC, Li K. An in Vivo miRNA Delivery System for Restoring Infarcted Myocardium. ACS Nano, 2019, 13(9):9880-9894  » PDF    » Article

Lindsay CD, Roth JG, LeSavage BL, Heilshorn SC. Bioprinting of stem cell expansion lattices. Acta Biomaterialia, 2019, 95:225-235  » PDF    » Article

Natarajan S, Foreman KM, Soriano MI, Rossen NS, Shehade H, Fregoso DR, Eggold JT, Krishnan V, Dorigo O, Krieg AJ, Heilshorn SC, Sinha S, Fuh KC, Rankin EB. Collagen remodeling in the hypoxic tumor-mesothelial niche promotes ovarian cancer metastasis. Cancer Research, 2019, 79(9):2271-2284  » PDF    » Article

Madl CM, LeSavage BL, Dewi RE, Lampe KJ, Heilshorn SC. Matrix remodeling enhances the differentiation capacity of neural progenitor cells in 3D hydrogels. Advanced Science, 2019, 6:1801716  » PDF    » Article

Atefyekta S, Pihl M, Lindsay CD, Heilshorn SC, Andersson, M. Antibiofilm elastin-like polypeptide coatings: functionality, stability, and selectivity. Acta Biomaterialia, 2019, 83:245-256  » PDF    » Article

Groll J, Burdick JA, Cho DW, Derby B, Gelinsky M, Heilshorn SC, et al. A definition of bioinks and their distinction from biomaterial inks. Biofabrication, 2019, 11:013001  » PDF    » Article

Krajina BA, Zhu A, Heilshorn SC, Spakowitz AJ. Active DNA olympic hydrogels driven by topoisomerase activity. Physical Review Letters, 2018, 121:148001  » PDF    » Article

Madl CM, Katz LM, Heilshorn SC. Tuning bulk hydrogel degradation by simultaneous control of proteolytic cleavage kinetics and hydrogel network architecture. ACS Macro Letters, 2018, 7:1302–1307  » PDF    » Article

Deming TJ, Klok HA, Armes SP, Becker ML, Champion JA, Chen EY, Heilshorn SC, et al. Polymers at the interface with biology. Biomacromolecules, 2018, 19(8):3151–3162  » PDF    » Article

George PM, Oh B, Dewi RE, Hua T, Cai L, Levinson A, Liang X, Krajina BA, Bliss TM, Heilshorn SC, Steinberg GK. Engineered stem cell mimics to enhance stroke recovery. Biomaterials, 2018, 178:63-72  » PDF    » Article

Wang H, Paul A, Nguyen D, Enejder A, Heilshorn SC. Tunable control of hydrogel microstructure by kinetic competition between self-assembly and crosslinking of elastin-like proteins. ACS Applied Materials & Interfaces, 2018, 10:21808-21815 » PDF    » Article

Lou J, Liu F, Lindsay C, Chaudhuri O, Heilshorn SC, Xia Y. Dynamic hyaluronan hydrogels with temporally modulated high injectability and stability using a biocompatible catalyst. Advanced Materials, 2018, 1705215  » PDF    » Article

Haugh M, Vaughan T, Madl CM, Raftery R, McNamara L, O'Brien F, Heilshorn SC. Investigating the interplay between substrate stiffness and ligand chemistry in directing mesenchymal stem cell differentiation within 3D macro-porous substrates. Biomaterials, 2018, 171:23-33  » PDF    » Article

Madl CM, Heilshorn SC, Blau H. Bioengineering strategies to accelerate stem cell therapeutics. Nature, 2018, 557:335-342 » PDF    » Article

LeSavage BL*, Suhar N*, Madl CM, Heilshorn SC. Production of elastin-like protein hydrogels for encapsulation and immunostaining of cells in 3D. Journal of Visualized Experiments, 2018, 135, doi:10.3791/57739 » PDF    » Article
*Denotes co-first authorship.

De la Zerda A, Kratochvil M, Suhar N, Heilshorn SC. Bioengineering strategies to probe T cell mechanobiology. APL Bioengineering, 2018, 2:021501 » PDF    » Article

Foster A, Dewi R, Cai L, Hou L, Strassberg Z, Alcazar C, Heilshorn SC, Huang N. Protein-engineered hydrogels enhance the survival of induced pluripotent stem cell-derived endothelial cells for treatment of peripheral arterial disease. Biomaterials Science, 2018, 6:614-622 » PDF    » Article

Madl CM and Heilshorn SC. Bio-orthogonal strategies for engineering extracellular matrices. Advanced Functional Materials, 2018, 28(11):1706046 » PDF    » Article

Nagy N, de la Zerda A, Kaber G, Johnson P, Hu K, Kratochvil M, Yadava K, Zhao W, Cui Y, Navarro G, Annes J, Wight T, Heilshorn SC, Bollyky P, Butte M. Hyaluronan content governs tissue stiffness in pancreatic islet inflammation. Journal of Biological Chemistry, 2018, 293(2):567-578 » PDF    » Article

Madl CM and Heilshorn SC. Engineering hydrogel microenvironments to recapitulate the stem cell niche. Annu. Rev. Biomed. Eng., 2018, 20:21-47 » PDF    » Article

Qin X, Chen H, Yang H, Wu H, Zhao X, Wang H, Chour T, Neofytou E, Ding D, Dalrup-Link H, Heilshorn SC, Li K, Wu JC. Photoacoustic imaging of embryonic stem cell-derived cardiomyocytes in living hearts with ultrasensitive semiconducting polymer nanoparticles. Advanced Functional Materials, 2018, 28(1):1704939 » PDF    » Article

Krajina B, Proctor A, Schoen AP, Spakowitz AJ, Heilshorn SC. Biotemplated synthesis of inorganic materials: an emerging paradigm for nanomaterial synthesis inspired by nature. Progress in Materials Science, 2018, 91:1-23 » PDF    » Article

Madl CM, LeSavage BL, Dewi R, Dinh C, Stowers R, Khariton M, Lampe K, Nguyen D, Chaudhuri O, Enejder A, Heilshorn SC. Maintenance of neural progenitor cell stemness in 3D hydrogels requires matrix remodelling. Nature Materials, 2017, 16:1233–1242 » PDF    » Article
*This article was featured on the cover of Nature Materials

Krajina B, Tropini C, Zhu A, DiGiacomo P, Sonnenburg JL, Heilshorn SC, Spakowitz AJ. Dynamic light scattering microrheology reveals multiscale viscoelasticity of polymer gels and precious biological materials. ACS Central Science, 2017, 3(21):1294-1303 » PDF    » Article

Paul A, Stührenberg M, Chen S, Rhee D, Lee W, Odom T, Heilshorn SC, Enejder A. Micro-and nano-patterned elastin-like polypeptide hydrogels for stem cell culture. Soft Matter, 2017, 13:5665-5675 » PDF    » Article

Steele A, Cai L, Truong V, Edwards B, Goldstone A, Eskandari A, Mitchell A, Marquardt L, Foster A, Cochran J, Heilshorn SC, Woo J. A novel protein-engineered hepatocyte growth factor analog released via a shear-thinning injectable hydrogel enhances post-infarction ventricular function. Biotechnol. Bioeng., 2017, 114:2379–2389 » PDF    » Article

Dubbin K, Tabet A, Heilshorn SC. Quantitative criteria to benchmark new and existing bio-inks for cell compatibility. Biofabrication, 2017, 9:044102 » PDF    » Article

Fernandes-Cunha G, Lee HJ, Kumar A, Kreymerman A, Heilshorn SC, Myung D. Immobilization of growth factors to collagen surfaces using pulsed visible light. Biomacromolecules, 2017, 18(10):3185–3196 » PDF    » Article

Wang H, Zhu D, Paul A, Cai L, Enejder A, Yang F, Heilshorn SC. Covalently adaptable elastin-like protein–hyaluronic acid (ELP–HA) hybrid hydrogels with secondary thermoresponsive crosslinking for injectable stem cell delivery. Advanced Functional Materials, 2017, 27:1605609  » PDF    » Article
*This article was featured on the cover of Advanced Functional Materials

DiMarco R*, Hunt D*, Dewi R, Heilshorn SC. Improvement of paracellular transport in the Caco-2 drug screening model using protein-engineered substrates. Biomaterials, 2017, 129:152-162 » PDF    » Article
*Denotes co-first authorship.

Zhu D*, Wang H*, Trinh P, Heilshorn SC, Yang F. Elastin-like protein-hyaluronic acid (ELP-HA) hydrogels with decoupled mechanical and biochemical cues for cartilage regeneration. Biomaterials, 2017, 127:132-140 » PDF    » Article
*Denotes co-first authorship.

Madl C, Heilshorn SC. Tyrosine-selective functionalization for bio-orthogonal cross-linking of engineered protein hydrogels. Bioconjugate Chemistry, 2017, 28(3):724-730  » PDF    » Article
*This article was featured on the cover of Bioconjugate Chemistry

Mascharak S, Benitez P, Proctor A, Madl C, Hu K, Dewi R, Butte M, Heilshorn SC. YAP-dependent mechanotransduction is required for proliferation and migration on native-like substrate topography. Biomaterials, 2017, 115:155-166 » PDF    » Article

Foster A, Marquardt L, Heilshorn SC. The diverse roles of hydrogel mechanics in injectable stem cell transplantation. Curr. Opin. Chem. Eng., 2017, 15:15-23 » PDF    » Article

Parisi-Amon A, Lo D, Montoro D, Dewi R, Longaker M, Heilshorn SC. Protein-nanoparticle hydrogels that self-assemble in response to peptide-based molecular recognition. ACS Biomater. Sci. Eng., 2017, 3(5):750-756  » PDF    » Article

Luan A, Zielins E, Wearda T, Atashroo D, Blackshear C, Raphel J, Brett E, Flacco J, Alyono M, Momeni A, Heilshorn SC, Longaker M, Wan D. Dynamic rheology for the prediction of surgical outcomes in autologous fat grafting. Plastic and Reconstructive Surgery, 2017, 140(3):517-524  » PDF    » Article

Cai L, Dewi R, Goldstone A, Cohen J, Steele A, Woo J, Heilshorn SC. Regulating stem cell secretome using injectable hydrogels with in situ network formation. Advanced Healthcare Materials, 2016, 5(21):2758-2764 » PDF    » Article

Dubbin K, Hori Y, Lewis K, Heilshorn SC. Dual-stage crosslinking of a gel-phase bioink improves cell viability and homogeneity for 3D bioprinting. Advanced Healthcare Materials, 2016, 5(19):2488-2492 » PDF    » Article
*This article was featured on the cover of Advanced Healthcare Materials

Madl CM, Heilshorn SC. Probing the metabolomics of stem cell differentiation with biomaterials. Chem, 2016, 1(2):192-194  » PDF    » Article

Marquardt L, Heilshorn SC. Design of injectable materials to improve stem cell transplantation. Current Stem Cell Reports, 2016, 2(3):207-220  » PDF    » Article

Quarta M, Brett J, DiMarco R, De Morree A, Boutet S, Chacon R, Gibbons M, Garcia V, Su J, Shrager J, Heilshorn SC, Rando T. An artificial niche preserves the quiescence of muscle stem cells and enhances their therapeutic efficacy. Nature Biotechnology, 2016, 34:752–759  » PDF    » Article

Haugh M, Heilshorn SC. Integrating concepts of material mechanics, ligand chemistry, dimensionality and degradation to control differentiation of mesenchymal stem cells. Curr. Opin. Solid State Mater. Sci., 2016, 20(4):171-179  » PDF    » Article

Raphel J, Holodniy M, Goodman SB, Heilshorn SC. Multifunctional coatings to simultaneously promote osseointegration and prevent infection of orthopaedic implants. Biomaterials, 2016, 84:301-314  » PDF    » Article

Madl CM, Katz L, Heilshorn SC. Bio-orthogonally crosslinked, engineered protein hydrogels with tunable mechanics and biochemistry for cell encapsulation. Advanced Functional Materials, 2016, 26(21):3612-3620  » PDF    » Article

Raphel J, Karlsson J, Galli S, Wennerberg A, Lindsay C, Haugh M, Pajarinen J, Goodman SB, Jimbo R, Andersson M, Heilshorn SC. Engineered protein coatings to improve the osseointegration of dental and orthopaedic implants. Biomaterials, 2016, 83:269-282  » PDF    » Article

Benitez P, Mascharak S, Proctor A, Heilshorn SC. Use of protein-engineered fabrics to identify design rules for integrin ligand clustering in biomaterials. Integrative Biology, 2016, 8:50-61  » PDF    » Article

Shamloo A, Mohammadaliha N, Heilshorn SC, Bauer AL. A comparative study of collagen matrix density effect on endothelial sprout formation using experimental and computational approaches. Annals of Biomedical Engineering, 2015, 44:929-941  » PDF    » Article

DiMarco R, Dewi RE, Bernal G, Kuo C, Heilshorn SC. Protein-engineered scaffolds for in vitro 3D culture of primary adult intestinal organoids. Biomaterials Science, 2015, 3:1376-85.  » PDF    » Article

Wang H, Heilshorn SC. Adaptable hydrogel networks with reversible linkages for tissue engineering. Advanced Materials, 2015, 27(25):3717-36.  » PDF    » Article

Romano N, Lampe K, Xu H, Ferreira M, Heilshorn SC. Microfluidic gradients reveal enhanced neurite outgrowth but impaired guidance within 3D matrices with high integrin ligand densities. Small, 2015, 11(6):722-730     » Article     » PDF

Ferreira MM, Dewi RE, Heilshorn SC. Microfluidic analysis of extracellular matrix-bFGF crosstalk on primary human myoblast chemoproliferation, chemokinesis, and chemotaxis. Integrative Biology, 2015, 7:569-579  » PDF    » Article

Madl CM, Heilshorn SC. Matrix interactions modulate neurotrophin-mediated neurite outgrowth and pathfinding. Neural Regeneration Research, 2015, 10(4):514-7 » PDF    » Article

Chang DT, Chai R, DiMarco R, Heilshorn SC, Cheng A. Protein-engineered hydrogel encapsulation for 3D culture of murine cochlea. Otology & Neurotology, 2015, 36(3):531-538      » Article     » PDF

Cai L, Dewi RE, Heilshorn SC. Injectable hydrogels with in situ double network formation enhance retention of transplanted stem cells. Advanced Functional Materials, 2015, 25(9):1344-51  » PDF    » Article

Romano N*, Madl CM*, Heilshorn SC. Matrix RGD ligand density and L1CAM-mediated Schwann cell interactions synergistically enhance neurite outgrowth. Acta Biomaterialia, 2015, 11(1):48–57  » PDF    » Article
*Denotes co-first authorship.

Huggins KNL, Schoen AP, Arunagirinathan MA, Heilshorn SC. Multi-site functionalization of protein scaffolds for bimetallic nanoparticle templating. Advanced Functional Materials, 2014, 24(48):7737–44  » PDF    » Article

Wang H, Cai L, Paul A, Enejder A, Heilshorn SC. Hybrid elastin-like polypeptide-polyethylene glycol (ELP-PEG) hydrogels with improved transparency and independent control of matrix mechanics and cell ligand density. Biomacromolecules, 2014, 15(9):3421–3428     » Article      » PDF

Greenwood-Goodwin M, Teasley ES, Heilshorn SC. Dual-stage growth factor release within 3D protein-engineered hydrogel niches promotes adipogenesis. Biomaterials Science, 2014, 2:1627-1639     » Article       » PDF
*This article was featured on the cover of Biomaterials Science

VanDersarl JJ, Mehraeen S, Schoen A, Heilshorn SC, Spakowitz A, Melosh NA. Rheology and simulation of 2-dimensional clathrin protein network assembly. Soft Matter, 2014, 10:6219-6227     » Article       » PDF

Mulyasasmita W, Cai L, Dewi R, Jha A, Ullmann SD, Luong RH, Huang NF, Heilshorn SC. Avidity-controlled hydrogels for injectable co-delivery of induced pluripotent stem cell-derived endothelial cells and growth factors. Journal of Controlled Release, 2014, 191:71-81     » Article       » PDF

Xu H, Ferreira MM, Heilshorn SC. Small-molecule axon-polarization studies enabled by a shear-free microfluidic gradient generator. Lab on a Chip, 2014, 14(12):2047-2056     » Article       » PDF

McLaughlin LM, Xu H, Carden SE, Fisher S, Reyes M, Heilshorn SC, Monack DM. A microfluidic-based genetic screen to identify microbial virulence factors that inhibit dendritic cell migration. Integrative Biology, 2014, 6:438-449     » Article       » PDF

Madl CM, Mehta M, Duda GN, Heilshorn SC, Mooney DJ. Presentation of BMP-2 mimicking peptides in 3D hydrogels directs cell fate commitment in osteoblasts and mesenchymal stem cells. Biomacromolecules, 2014, 15(2):445-55     » Article       » PDF

Cai L, Dinh CB, Heilshorn SC. One-pot synthesis of elastin-like polypeptide hydrogels with grafted VEGF-mimetic peptides. Biomaterials Science, 2014, 2:757-765
    » Article       » PDF

Cai L, Heilshorn SC. Designing ECM-mimetic materials using protein engineering. Acta Biomaterialia, 2014, 10(4):1751-1760     » Article       » PDF

DiMarco R, Su J, Yan K, Dewi R, Kuo CJ, Heilshorn SC. Engineering of three-dimensional microenvironments to promote contractile behavior in primary intestinal organoids. Integrative Biology, 2014, 6(2):127-142     » Article       » PDF
*This article was featured on the cover of Integrative Biology

Mulyasasmita W, Cai L, Hori Y, Heilshorn SC. Avidity-controlled delivery of angiogenic peptides from injectable molecular-recognition hydrogels. Tissue Engineering, Part A, 2014, 20(15-16):2102-14     » Article      » PDF
Schoen AP, Huggins KNL, Heilshorn SC. Engineered clathrin nanoreactors provide tunable control over gold nanoparticle synthesis and clustering. Journal of Materials Chemistry B, 2013, 1:6662-6669     » Article      » PDF

Huang NF, Dewi RE, Okogbaa J, Lee JC, Rufaihah A, Heilshorn SC, Cooke JP. Chemotaxis of human induced pluripotent stem cell-derived endothelial cells. American Journal of Translational Research, 2013, 5(5):510-520     » Article       » PDF

Shamloo A, Manchandia M, Ferreira M, Mani M, Nguyen C, Jahn T, Weinberg K, Heilshorn SC. Complex chemoattractive and chemorepellent Kit signals revealed by direct imaging of murine mast cells in microfluidic gradient chambers. Integrative Biology, 2013, 5:1076-1085     » Article      » PDF

Chung C, Pruitt BL, Heilshorn SC. Spontaneous cardiomyocyte differentiation of mouse embryoid bodies regulated by hydrogel crosslink density. Biomaterials Science, 2013, 1(10):1082-1090     » Article       » PDF

Schoen AP, Cordella N, Mehraeen S, Arunagirinathan MA, Spakowitz AJ, Heilshorn SC. Dynamic remodeling of disordered protein aggregates is an alternative pathway to achieve robust self-assembly of nanostructures. Soft Matter, 2013, 9(38):9137-9145      » Article       » PDF
Schoen AP, Hommersom B, Heilshorn SC, Leunissen ME. Tuning colloidal association with specific peptide interactions. Soft Matter, 2013, 9(29):6781-6785     » Article       » PDF
Lampe KJ, Antaris AL, Heilshorn SC. Design of 3D engineered protein hydrogels for tailored control of neurite growth. Acta Biomaterialia, 2013, 9:5590-5599     » Article       » PDF
Parisi-Amon A, Mulyasasmita W, Chung C, Heilshorn SC. Protein-engineered injectable hydrogel to improve retention of transplanted adipose-derived stem cells. Advanced Healthcare Materials, 2013, 2:428-432     » Article   » PDF
Xu H, Heilshorn SC. Microfluidic investigation of BDNF enhanced neural stem cell chemotaxis in CXCL12 gradients. Small, 2013, 9:585-595     » Article   » PDF
Benitez P, Sweet JA, Fink H, Chennazhi KP, Nair SK, Enejder A, Heilshorn SC. Sequence-specific crosslinking of electrospun elastin-like protein preserves bioactivity and native-like mechanics. Advanced Healthcare Materials, 2013, 2:114-118     » Article   » PDF
Chung C, Lampe K, Heilshorn SC. Tetrakis (hydroxyl methyl) phosphonium chloride as a covalent crosslinking agent for cell encapsulation within protein-based hydrogels. Biomacromolecules, 2012, 13:3912-3916     » Article       » PDF
Chung C, Anderson E, Reijo Pera R, Pruitt BL, Heilshorn SC. Hydrogel crosslinking density regulated temporal contractility of human embryonic stem cell-derived cardiomyocytes in 3D cultures. Soft Matter, 2012, 8:10141-10148      » Article       » PDF
DiMarco RL, Heilshorn SC. Multifunctional materials through modular protein engineering. Advanced Materials, 2012, 24:3923-3940      » Article       » PDF
Sengupta D, Gilbert P, Johnson K, Blau HM, Heilshorn SC. Protein-engineered biomaterials to generate human skeletal muscle mimics. Advanced Healthcare Materials, 2012, 1:785-789      » Article   » PDF
Yan KS, Chia LA, Li X, Ootani A, Su J, Lee JY, Su N, Luo Y, Heilshorn SC, Amieva MR, Sangiorgi E, Capecchi MR, Kuo C. The intestinal stem cell markers Bmi1 and Lgr5 identify two functionally distinct populations. Proceedings of the National Academy of Sciences USA, 2012, 109:466-471      » Article       » PDF
Raphel J, Parisi-Amon A, Heilshorn SC. Photoreactive elastin-like proteins for use as versatile bioactive materials and surface coatings. Journal of Materials Chemistry, 2012, 22:19429-19437      » Article       » PDF
*This is a peer-reviewed article for a special issue on Materials for Biosurfaces
Lampe KJ, Heilshorn SC. Building stem cell niches from the molecule up through engineered peptide materials. Accepted for publication, Neuroscience Letters, 2012, 519:138-146      » Article       » PDF
*This is a peer-reviewed, invited article for a special issue on Bioengineered Approaches to Nervous System Repair
Aguado BA, Mulyasasmita W, Su J, Lampe KJ, Heilshorn SC. Improving viability of stem cells during syringe needle flow through the design of hydrogel cell carriers. Tissue Engineering Part A, 2012, 18:806-815     » Article       » PDF
Shamloo A, Xu H, Heilshorn SC. Mechanisms of VEGF-induced path-finding by endothelial sprouts in biomaterials. Tissue Engineering Part A, 2012, 18:320-330
     » Article       » PDF
Schoen AP, Schoen DT, Huggins KN, Arunigirinathan MA, Heilshorn SC. Template engineering through epitope recognition: A modular, biomimetic strategy for inorganic nanomaterial synthesis. Journal of the American Chemical Society, 2011, 133:18202-18207     » Article       » PDF
Mulyasasmita W, Lee JS, Heilshorn SC. Molecular-level engineering of protein physical hydrogels for predictive sol-gel phase behavior. Biomacromolecules, 2011, 12:3406-3411     » Article       » PDF
Nevill JT, Mo A, Cord BJ, Palmer TD, Poo MM, Lee LP, Heilshorn SC. Vacuum soft lithography for one-step patterning of multiple biomolecules to direct neuronal polarization. Soft Matter, 2011, 7:343-347     » Article       » PDF
*This article was featured on the cover of Soft Matter
Romano N, Sengupta D, Chung C, Heilshorn S. Protein-engineered biomaterials: Nanoscale mimics of the extracellular matrix. Biochimica et Biophysica Acta, 2011, 1810:339-349     » Article       » PDF
*This is a peer-reviewed, invited article for a special issue on Emerging Applications of Nanotechnologies in Biomedicine
Kuhnert F, Mancuso MR, Shamloo A, Wang HT, Choksi V, Florek M, Su H, Fruttiger M, Young WL, Heilshorn SC, Kuo CJ. Essential regulation of CNS angiogenesis by the orphan G protein-coupled receptor GPR124. Science, 2010, 330:985-989     » Article       » PDF
Schoen DT*, Schoen AP*, Hu L, Kim HS, Heilshorn SC, Cui Y. High speed water sterilization using one dimensional nanostructures. Nano Letters, 2010, 10:3628-3632
     » Article       » PDF
Shamloo A, Heilshorn SC. Matrix density mediates polarization and lumen formation of endothelial sprouts in VEGF gradients. Lab on a Chip, 2010, 10:3061-3068      » Article       » PDF
*This article was featured on the cover of Lab on a Chip
Shelly M, Lim BK, Cancedda L, Heilshorn SC, Gao H, Poo MM. Local and long-range reciprocal regulation of cAMP and cGMP in axon/dendrite formation. Science, 2010, 327:547-552     » Article       » PDF
Sengupta D, Heilshorn SC. Protein-engineered biomaterials: Highly tunable tissue engineering scaffolds. Tissue Engineering Part B: Reviews, 2010, 16:285-293     » Article       » PDF
*This article was featured on the cover of Tissue Engineering Part B: Reviews
Straley KS, Wong Po Foo C, Heilshorn SC. Biomaterial design strategies for the treatment of spinal cord injuries. Journal of Neurotrauma, 2010, 27:1-19     » Article       » PDF
Wong Po Foo C, Lee JS, Mulyasasmita W, Parisi-Amon A, Heilshorn SC. Two-component protein-engineered physical hydrogels for cell encapsulation. Proceedings of the National Academy of Sciences USA, 2009, 106:22067-22072     » Article       » PDF
Straley KS and Heilshorn SC. Dynamic, three-dimensional pattern formation within enzyme-responsive hydrogels. Advanced Materials, 2009, 21:4148-4152       » Press release     » Article       » PDF
*This article was featured on the cover of Advanced Materials
Straley KS and Heilshorn SC. Design and adsorption of modular engineered proteins to prepare customized, neuron-compatible coatings. Frontiers in Neuroengineering, 2009, 2:9     » Article       » PDF
Wilson RJ, Hu W, Wong Po Foo C, Koh AL, Gaster RS, Earhart CM, Fu A, Heilshorn SC, Sinclair R, Wang SX. Formation and properties of magnetic chains for 100 nm nanoparticles used in separations of molecules and cells. Journal of Magnetism and Magnetic Materials, 2009, 321:1452-1458     » Article       » PDF
Wang S, Wong Po Foo C, Warrier A, Poo MM, Heilshorn SC, Zhang X. Gradient lithography of engineered proteins to fabricate 2D and 3D cell culture environments. Biomedical Microdevices, 2009, 11:1127-1134     » Article       » PDF
Straley K, Heilshorn SC. Independent tuning of multiple biomaterial properties using protein engineering.  Soft Matter, 2009, 5:114-124     » Article       » PDF
Shamloo A, Ma N, Poo MM, Sohn L, Heilshorn SC. Endothelial cell polarization and chemotaxis in a microfluidic device.  Lab on a Chip, 2008, 8:1292-1299     » Article       » PDF
Shelly M, Cancedda L, Heilshorn S, Sumbre G, Poo M.  LKB1/STRAD promotes axon initiation during neuronal polarization.  Cell, 2007, 129:565-577     » Article       » PDF
Carrico IS, Maskarinec SA, Heilshorn SC, Mock ML, Liu JC, Nowatzki PJ, Franck C, Ravichandran G, Tirrell D.  Lithographic patterning of photoreactive cell-adhesive proteins.  Journal of the American Chemical Society, 2007, 129:4874-4875     » Article       » PDF
Heilshorn SC, Liu JC, DiZio KA, Tirrell DA. Cell-binding domain context affects cell behavior on engineered proteins.  Biomacromolecules, 2005, 6:318-323     » Article       » PDF
Liu JC, Heilshorn SC, Tirrell DA.  Comparative cell response to artificial extracellular matrix proteins containing the RGD and  CS5 cell-binding domains.  Biomacromolecules, 2004, 5:497-504     » Article       » PDF
Heilshorn SC, DiZio KA, Welsh ER, Tirrell DA.  Endothelial cell adhesion to the fibronectin CS5 domain in artificial extracellular matrix proteins.  Biomaterials, 2003, 24:4245-4252     » Article       » PDF
Glenn RW, Sine MR, Evans MD, Carethers ME, Heilshorn SC. "Liquid personal cleansing compositions which contain a complex coacervate for improved sensory perception", U.S. patents #6,028,043 (2000), #5,932,528 (1999), #5,858,938 (1999), #5,716,920 (1998). Assignee: The Procter Gamble Company
Lindsay CD, Heilshorn SC. (2019) "Shear Thinning Hydrogel-based 3D Tissue Modelling" Ed. Cho DW, Biofabrication and 3D Tissue Modeling, Cambridge, UK, Royal Society of Chemistry.
Cai L, Heilshorn SC. (2018) "Designing Protein-Engineered Biomaterials for Stem Cell Therapy" Eds. Huang N, L'Heureux N, Li S, Engineering Stem Cells for Tissue Regeneration, Hackensack, New Jersey, World Scientific.
Benitez P, Heilshorn SC. (2013) "Microfluidic Devices for Quantifying the Role of Soluble Gradients in Early Angiogenesis." Ed. Reinhart-King CA, Mechanical and Chemical Signaling in Angiogenesis, Heidelberg, Germany, Springer.
Raphel J, Parisi-Amon AP, Heilshorn SC. (2012) "Protein-Engineered Hydrogels." Eds. Taubert A, Mano J, Rodriquez-Cabello JC, Biomaterials Surface Science, Mannheim, Germany, Wiley-VCH Verlag.
Parisi-Amon A, Heilshorn SC. (2012) "Engineered Protein Biomaterials." Eds. Bronzino JD, Peterson DR, Section Eds. Fisher JP, Mikos AG, Biomedical Engineering Handbook, 4th Edition, Volume 4, Boca Raton, FL, CRC Press, Taylor & Francis Group.
Greenwood-Goodwin M, Heilshorn SC. (2012) "Hydrogels from Protein Engineering." Ed. Mano JF, Biomimetic Approaches for Biomaterials Development. Mannheim, Germany, Wiley-VCH Verlag.
Mulyasasmita W, Heilshorn SC. (2011) "Protein-Engineered Biomaterials: Synthesis and Characterization." Eds. Ducheyne P, Healy K, Hutmacher DW, Grainger DW, Kirkpatrick CJ, Comprehensive Biomaterials. Oxford, UK, Elsevier Science.
Wong Po Foo C, Heilshorn SC. (2010) "Protein Engineered Biomaterials." Eds. Park SJ, Cochran JR, Protein Engineering. Boca Raton, FL, CRC Press, Taylor & Francis Group.